Invitrogen™ Anza™ DNA End Repair Kit, 20 Rxns

The Invitrogen™ Anza™ DNA End Repair Kit is a ready-to-use reagent system that converts DNA fragments with cohesive ends into blunt-ended, 5′-phosphorylated DNA suitable for blunt-end ligation. The pre-mixed Anza™ DNA End Repair Mix contains T4 DNA polymerase, Klenow Fragment, and T4 Polynucleotide Kinase (PNK) to streamline workflows by enabling simultaneous end polishing and phosphorylation. The Anza™ 10X End Repair Buffer contains ATP and dNTPs to ensure optimal reaction conditions.

Key Features and Benefits

• Pre-mixed Enzyme Mix – Reduces pipetting and setup time
• Simultaneous Blunting and Phosphorylation – Efficient 2-in-1 functionality
• Fast Reaction Time – Complete in just 15 minutes at room temperature
• Anza-Compatible – Directly compatible with Anza™ restriction enzyme workflows
• High Reliability – Supports seamless integration into cloning protocols

Specifications

Kit Contents

Applications

• Blunt-end cloning
• End repair of DNA fragments after restriction digestion
• Preparation of DNA for ligation into blunt-end vectors
• Polishing PCR products or sheared DNA prior to cloning
• Integration with Anza™ modular cloning systems

The Invitrogen™ Anza™ DNA End Repair Kit delivers efficient, high-performance end polishing and phosphorylation of DNA in a fast, 15-minute protocol. Ideal for cloning workflows requiring blunt-end ligation, this kit minimizes hands-on time while maximizing reliability and compatibility within the Anza™ Cloning System.