Invitrogen™ SuperScript™ IV First-Strand Synthesis System

The Invitrogen™ SuperScript™ IV First-Strand Synthesis System for RT-PCR delivers fast, robust, and reliable cDNA synthesis from purified total RNA or poly(A)+ RNA. Available in formats with or without ezDNase enzyme, the system provides flexibility for applications requiring genomic DNA removal while ensuring reproducibility and sensitivity in downstream PCR and qPCR assays.

At its core is SuperScript™ IV Reverse Transcriptase, an engineered M-MLV mutant with enhanced thermostability, processivity, and inhibitor resistance, while maintaining low RNase H activity. The system achieves cDNA synthesis in as little as 10 minutes, significantly faster than SuperScript™ III–based systems, while producing full-length cDNA up to 12.3 kb.

The ezDNase-enabled version simplifies and accelerates genomic DNA removal, eliminating the variability introduced by traditional DNase treatments and reducing RNA loss.

Key Features

• Faster Reactions – Complete cDNA synthesis in ~10 minutes (reduced from >50 min).
• Improved Inhibitor Resistance – Reliable performance across diverse, inhibitor-rich sample types.
• Enhanced Processivity – Produces long cDNA products (up to 12.3 kb).
• Specific & Robust – High yields of full-length cDNA with reduced RNase activity.
• Simplified DNA Removal – Versions with ezDNase enzyme streamline removal of genomic DNA.
• Flexible Formats – Kits available for 50 or 200 reactions, with or without ezDNase.

Applications

• First-strand cDNA synthesis from total RNA or poly(A)+ RNA
• RT-PCR and qRT-PCR
• Gene expression analysis
• Detection of low-abundance transcripts
• cDNA synthesis in the presence of inhibitors
• Studies requiring long cDNA products (up to 12.3 kb)

Technical Specifications

Kit Configurations & Contents

The Invitrogen™ SuperScript™ IV First-Strand Synthesis System represents a next-generation reverse transcription solution, offering unparalleled speed, sensitivity, and reproducibility. With the option of integrated ezDNase treatment, it provides the most efficient workflow for RT-PCR and qPCR applications, particularly when working with challenging samples prone to inhibitors or genomic DNA contamination.