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Invitrogen™ SYTO™ Green Fluorescent Nucleic Acid Stains
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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Gel Stain | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ SYTO™ Green Fluorescent Nucleic Acid Stains are a collection of cell-permeant nucleic acid dyes that bind selectively to DNA or RNA, emitting bright green fluorescence upon interaction. These dyes are widely used for live-cell and fixed-cell imaging, bacterial staining, flow cytometry, and nucleic acid quantification.
Unlike traditional nuclear stains like DAPI or Hoechst, SYTO dyes permeate both live and dead cells and can stain nuclear, cytoplasmic, and mitochondrial nucleic acids. The variety of SYTO dyes provides flexibility in selecting a dye based on cell type, nucleic acid selectivity, fluorescence intensity, and spectral compatibility with commonly used fluorophores such as FITC, GFP, and Alexa Fluor™ 488.
Key Features and Benefits
- Cell-Permeant: Penetrates live cells, bacteria, and fixed tissues
- High Fluorescence Enhancement: Quantum yields >0.4 when bound to nucleic acids
- Broad Applicability: RNA and DNA staining in bacteria, mammalian cells, and exosomes
- Spectrally Compatible: Compatible with FITC/GFP filters and flow cytometers
- Dye Variety: 10+ green dyes available individually or in a sampler kit for assay optimization
SYTO™ Green Fluorescent Dyes – Comparison and Applications
| Dye Type | Ex/Em (DNA) | Ex/Em (RNA) | Primary Application |
|---|---|---|---|
| SYTO 9 Green | 485/498 nm | 486/501 nm | Live/dead staining of Gram+ and Gram– bacteria; part of LIVE/DEAD BacLight kits |
| SYTO 11 Green | 508/527 nm | 510/530 nm | Time-lapse microscopy; cleavage orientation in dividing cerebral cortex cells |
| SYTO 12 Green | 499/522 nm | 500/519 nm | General RNA and DNA staining in live mammalian cells |
| SYTO 13 Green | 488/509 nm | 491/514 nm | Glutamate-induced necrosis detection in cerebellar neurons (paired with PI) |
| SYTO 14 Green | 517/549 nm | 521/547 nm | RNA granule transport tracking in live neurons |
| SYTO 16 Green | 488/518 nm | 494/525 nm | Apoptosis detection in thymocytes; live/dead discrimination in COS-7 cells |
| SYTO 21 Green | 494/517 nm | — | General nuclear DNA staining; strong signal in live-cell imaging |
| SYTO 24 Green | 490/515 nm | — | DNA staining in eukaryotic cells; used in high-content screening |
| SYTO BC Green | 485/500 nm | 487/504 nm | Bacterial cell enumeration; used in Bacteria Counting Kit (Cat. No. B7277) |
| SYTO RNASelect | ~490/530 nm | ~490/530 nm | RNA-selective dye; exosome staining, RNA cargo visualization, minimal DNA fluorescence |
Applications
- Live/Dead Bacterial Viability Assays (SYTO 9, BC)
- Live-Cell Imaging (SYTO 11–16, 21, RNASelect)
- Flow Cytometry Nucleic Acid Detection (All dyes)
- Apoptosis & Necrosis Studies (SYTO 13, SYTO 16)
- Exosome RNA Labeling (SYTO RNASelect)
- RNA Granule and Cytoplasmic RNA Tracking (SYTO 14, RNASelect)
- DNA Microarrays & High-Content Screening (SYTO 24, 21)
- Multiplex Fluorescence Microscopy (FITC/GFP channel compatible)
Storage & Handling
- Store all SYTO Green dyes at –5°C to –30°C
- Protect from light
- Dilute in PBS or any physiological buffer (pH 7.0–8.0) immediately before use
Invitrogen™ SYTO™ Green Fluorescent Nucleic Acid Stains are versatile, high-sensitivity dyes for nucleic acid detection in a wide range of live and fixed cells, tissues, and bacteria. Their strong green fluorescence and wide compatibility with common optical systems make them essential tools for modern molecular and cell biology workflows.
- Description: SYTO 12 Green Fluorescent Nucleic Acid Stain, 4 mM Solution in DMSO, 315 µL SYTO 13 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO, 250 µL SYTO 14 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO, 250 µL SYTO 16 Green Fluorescent Nucleic Acid Stain, 1 mM Solution in DMSO, 250 µL SYTO 21 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO, 250 µL SYTO 24 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO, 250 µL SYTO BC Green Fluorescent Nucleic Acid Stain, 100 µL SYTO RNASelect Green Fluorescent Cell Stain, 5 mM Solution in DMSO, 100 µL 1 kit SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1: SYTO dyes 11, 14, 16, 21, 24 (50 μL each) SYTO 9 Green Fluorescent Nucleic Acid Stain SYTO 9 Green Fluorescent Nucleic Acid Stain, 100 µL