Invitrogen™ eBioscience™ HLA-DR Monoclonal Antibody (LN3), PE-Cyanine7

Invitrogen™ eBioscience™ HLA-DR Monoclonal Antibody (LN3), PE-Cyanine7

The Invitrogen™ eBioscience™ HLA-DR Monoclonal Antibody (LN3) is a reliable reagent conjugated with PE-Cyanine7, optimized for detecting HLA-DR, a human MHC class II antigen. This antibody is specifically designed for flow cytometry applications and provides precise results with minimal background interference.

Key Features

1. Target Molecule (HLA-DR):
   • HLA-DR is a major histocompatibility complex (MHC) class II molecule expressed on antigen-presenting cells (APCs), including B cells, monocytes, macrophages, dendritic cells, and activated T cells.
   • It plays a critical role in antigen presentation to CD4+ T lymphocytes, initiating adaptive immune responses (1).
2. Conjugate:
   • PE-Cyanine7, a tandem fluorochrome, provides strong fluorescence signals with an excitation maximum of 569 nm and an emission maximum of 780 nm, compatible with blue, green, and yellow-green lasers (2).
3. Applications:
   • Designed for flow cytometric analysis of HLA-DR expression in human peripheral blood and tissue samples.
   • Useful in studies involving immune response, transplant compatibility, and immune system disorders (3).
4. Format and Purification:
   • Purified via affinity chromatography, supplied in PBS with 0.2% BSA and 0.09% sodium azide, ensuring stability and reliability (4).

Specifications

Applications

1. Flow Cytometry:
   • Accurate detection and quantification of HLA-DR+ antigen-presenting cells, including dendritic cells, B cells, and monocytes.
2. Immune Response Studies:
   • Investigation of antigen presentation pathways and adaptive immune responses.
3. Transplant Research:
   • HLA-DR expression profiling to assess compatibility in bone marrow and organ transplants.
4. Disease Research:
   • Study HLA-DR involvement in autoimmune diseases, cancer, and infectious diseases.
5. Immunopeptidomics:
   • Facilitates identification of MHC class II-bound peptides using advanced analytical techniques like mass spectrometry (5).

Handling and Storage

• Storage Conditions: Store at 4°C in the dark to maintain dye stability. Do not freeze to prevent damage to the antibody and fluorochrome.
• Light Sensitivity: Protect from light as PE-Cyanine7 is sensitive to photo-induced oxidation.
• Sample Fixation: Compatible with IC Fixation Buffer (Product #00-822-49) for sample storage post-staining.

Advantages

1. High Fluorescence Intensity:
   • PE-Cyanine7 provides a bright and stable signal, enhancing sensitivity for detecting low-expression markers.
2. Specificity:
   • The LN3 clone ensures specific binding to HLA-DR, minimizing background noise.
3. Validated Applications:
   • Pre-titrated for optimal use in flow cytometry, ensuring reproducibility and reliability.

Target Information

HLA-DR is a heterodimeric transmembrane protein composed of an alpha chain (36 kDa) and a beta chain (27 kDa). It binds processed peptides intracellularly and presents them to CD4+ T cells. Polymorphisms in the beta chain define peptide-binding specificities and are critical for transplant compatibility and immune recognition (6).

References

1. Mellins, E. D., et al. "HLA-DR and disease associations: mechanisms of antigen presentation." Current Opinion in Immunology 14.1 (2002): 28-35.
2. Invitrogen™ Datasheet for HLA-DR Monoclonal Antibody, PE-Cyanine7 (Product #25-9956-42).
3. Stern, L. J., et al. "Structural biology of the MHC class II antigen presentation pathway." Annual Review of Immunology 14.1 (1996): 365-396.
4. Ziegler, A., et al. "HLA class II-associated diseases with a focus on HLA-DR: molecular background and clinical relevance." HLA 93.1 (2019): 5-14.
5. Hunt, D. F., et al. "Characterization of peptides bound to the class II MHC molecule HLA-DR1 by mass spectrometry." Science 256.5065 (1992): 1817-1820.
6. Morris, G. P., et al. "The importance of defining the peptide-binding specificities of MHC molecules in transplantation." Nature Reviews Immunology 12.6 (2012): 433-440.

The Invitrogen™ eBioscience™ HLA-DR Monoclonal Antibody (LN3), PE-Cyanine7 is an essential tool for immunological research, particularly in antigen presentation, transplant compatibility, and disease studies. Its robust performance and bright fluorophore make it highly suitable for sensitive flow cytometry applications.