Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody (HI100), FITC

Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody (HI100), FITC

Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody is designed for use in flow cytometry applications. The antibody detects CD45RA, a marker for subsets of human leukocytes, using a high-quality FITC (fluorescein isothiocyanate) conjugate for fluorescence detection.

Key Features

1. Target Information:
   • Reacts with CD45RA, a variant of the CD45 antigen expressed on subsets of human leukocytes.
   • Marker for naive T cells and subsets of B cells.
2. Applications:
   • Optimized for flow cytometry.
   • Pre-titrated and tested for use on human peripheral blood cells.
3. Fluorescence Detection:
   • Conjugated to FITC with Excitation/Emission Max of 488/519 nm.
   • Designed for use with blue laser (488 nm) in flow cytometry.
4. Host and Isotype:
   • Produced in mouse, IgG2b kappa.
5. Purity and Formulation:
   • Purified via affinity chromatography.
   • Provided as a liquid formulation in PBS (pH 7.2) with 0.2% BSA and 0.09% sodium azide.

Specifications

Applications

1. Flow Cytometry:
   • Detection and analysis of human CD45RA+ leukocytes.
   • Staining of naive T cells and subsets of B cells in human peripheral blood.
2. Immunophenotyping:
   • Identification of immune cell populations in research studies.
3. Immune Response Research:
   • Used to study T cell maturation and immune function.

Preparation and Handling

• Storage: Store at 4°C in the dark. Avoid freezing as it may compromise performance.
• Light Sensitivity: Protect from light to maintain dye fluorescence integrity.
• Fixation Compatibility: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days.

Additional Notes

• Light Source Compatibility: Designed for excitation with blue laser (488 nm) and emission detection at 519 nm.
• Sample Fixation: Recommended fixation buffers include Invitrogen IC Fixation Buffer (Product # 00-822-49).
• Panel Builder: Use Invitrogen's Flow Cytometry Panel Builder for designing customized multicolor panels.

The Invitrogen™ eBioscience™ CD45RA Monoclonal Antibody (HI100), FITC is a reliable, high-quality reagent for identifying and characterizing CD45RA+ cells in flow cytometry. Its FITC conjugation ensures consistent fluorescence performance, making it an essential tool for immune cell analysis in research studies.