Invitrogen™ eBioscience™ CD4 Monoclonal Antibody (SK3 (SK-3)), PerCP-eFluor™ 710

Invitrogen™ eBioscience™ CD4 Monoclonal Antibody (SK3), PerCP-eFluor™ 710

The Invitrogen™ eBioscience™ CD4 Monoclonal Antibody (SK3) is a high-quality reagent designed for the detection of CD4 antigen in human and mouse samples. It is conjugated with PerCP-eFluor™ 710, offering superior fluorescence performance for flow cytometry applications.

Key Features

1. Target Information:
   • Recognizes CD4, a 59-kDa cell surface receptor expressed by T helper cells, monocytes, macrophages, and dendritic cells.
   • Blocks HIV binding and mixed lymphocyte reaction by targeting CD4.
2. Applications:
   • Specifically tested for flow cytometric analysis.
   • Used to study T helper cell populations, HIV research, and immune response.
3. Fluorescence Detection:
   • Conjugated with PerCP-eFluor™ 710 for excitation at 488 nm and emission at 710 nm.
   • Recommended filter: 710/50 bandpass; alternative: 695/40 bandpass.
4. Purity and Format:
   • Purified via affinity chromatography.
   • Provided as a liquid in PBS (pH 7.2) with 0.2% BSA and 0.09% sodium azide.

Specifications

Applications

1. Flow Cytometry:
   • Identification and analysis of CD4+ T helper cells.
   • Immune system studies in normal and diseased states.
2. HIV Research:
   • Study of CD4 as a receptor for HIV binding and entry.
3. Immunophenotyping:
   • Characterization of monocytes, macrophages, and dendritic cells.
4. Tissue-Level Studies:
   • Analysis of CD4 expression in lymphoid and non-lymphoid tissues, including thymus and spleen.

Preparation and Handling

• Storage: Store at 4°C in the dark; do not freeze to prevent damage to the antibody and fluorochrome.
• Fixation Compatibility: Samples can be fixed using Invitrogen IC Fixation Buffer (Product # 00-822-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days without significant loss of brightness.
• Light Sensitivity: Protect from light to maintain fluorochrome stability.

Advantages of PerCP-eFluor™ 710

• High sensitivity for detection of low-expression antigens.
• Minimal spectral overlap with common fluorochromes, improving multicolor panel compatibility.
• Compatible with blue laser excitation (488 nm).

Additional Notes

• Filter Requirements: Ensure the flow cytometer is configured to detect PerCP-eFluor™ 710 fluorescence using the appropriate bandpass filter.
• Titration: Pre-titrated for optimal performance; 5 µL (0.06 µg) per test is recommended.

The Invitrogen™ eBioscience™ CD4 Monoclonal Antibody (SK3), PerCP-eFluor™ 710 is an excellent reagent for flow cytometry, offering reliable detection of CD4+ cells. Its superior fluorescence, high specificity, and robust performance make it an essential tool for immunological studies, particularly in T cell characterization and HIV research.