Gibco™ DPBS (10X), no calcium, no magnesium

Gibco™ DPBS (10X) is a sterile, concentrated, balanced salt solution designed for a wide range of cell culture applications, including cell washing, reagent preparation, and sample transport. This formulation is calcium- and magnesium-free, making it ideal for cell dissociation protocols, particularly those involving chelating agents such as EDTA or trypsin.

Key Features

• Calcium- and magnesium-free – Ideal for rinsing cells before enzymatic dissociation
• 10X concentrated formulation – Requires dilution and pH adjustment prior to use
• No phenol red – Suitable for applications sensitive to color indicators
• Sterile-filtered and animal origin-free (AOF) – Ensures high quality and safety for cell culture workflows
• ISO 13485 and cGMP-compliant – Manufactured under strict quality standards

Specifications

Applications

• Washing cells prior to enzymatic dissociation (e.g., with trypsin or EDTA)
• Sample handling and transportation of tissues or cells
• Dilution of cells for counting
• Buffer component in reagent formulation
• Use in systems requiring low ion concentrations to avoid calcium/magnesium interference

Storage & Handling

• Storage: 15°C to 30°C
• Shelf Life: 36 months from date of manufacture
• Shipping: Room temperature
• Preparation: Requires pH adjustment and filtration when diluted to working strength

Gibco™ DPBS (10X), no calcium, no magnesium provides a flexible and consistent buffer system for critical cell culture workflows, particularly in preparatory steps before enzymatic cell detachment. Its sterile, high-quality formulation ensures compatibility with sensitive biological systems and GMP-regulated lab environments.