Mabtech™ ELISpot™ Plus: Human IL-1β (ALP) ELISA Kit

The Mabtech™ ELISpot™ Plus: Human IL-1β (ALP) ELISA Kit is designed for the enumeration of human IL-1β-secreting cells using the ELISpot assay. This kit detects both the pro-form and active form of IL-1β with high specificity, making it ideal for research into immune responses, inflammation, and cytokine signaling.

Intended Use

• Purpose:
  • Enumerate cells secreting human IL-1β using the ELISpot assay.
• Usage:
  • For research use only. Not for use in diagnostic procedures.

Product Details

• Product Name: ELISpot Plus: Human IL-1β (ALP)
• Application: ELISpot
• Analyte: IL-1β
• Reactivity: Human
• Specificity:
  • Based on a matched pair of monoclonal antibodies (mAbs) specific for human IL-1β (pro-form and active form).
• Cross-Reactivity:
  • The mAbs do not cross-react with human IL-1α.

Shipping and Storage

• Shipping Conditions:
  • Shipped at ambient temperature.
• Storage Conditions:
  • Store antibodies, enzyme conjugate, and substrate at 4–8°C upon receipt.
  • Plates can be kept at room temperature.
• Shelf Life:
  • At least 18 months from the date of receipt.

Key Features

1. Specific Detection:
   • Detects both pro-form and active forms of IL-1β with high specificity.
2. Cross-Reactivity Information:
   • mAbs do not cross-react with IL-1α, ensuring precise targeting of IL-1β.
3. Ease of Storage:
   • Room temperature storage for plates simplifies laboratory logistics.
4. Long Shelf Life:
   • Valid for at least 18 months, ensuring reliability over extended periods.

Applications

• Immune Research:
  • Enumerate IL-1β-secreting cells in immune response studies.
• Inflammation Studies:
  • Assess IL-1β secretion in inflammatory diseases and conditions.
• Cytokine Profiling:
  • Use in cytokine profiling assays to study immune regulation.
• Therapeutic Development:
  • Evaluate immune responses in preclinical and clinical therapeutic studies.

Advantages

1. Matched mAbs:
   • Specificity for human IL-1β ensures accurate results.
2. Versatility:
   • Suitable for research into both innate and adaptive immune responses.
3. Precision:
   • Avoids cross-reactivity with IL-1α, reducing false positives.
4. Convenience:
   • Plates stored at room temperature ease storage requirements.

The Mabtech™ ELISpot™ Plus: Human IL-1β (ALP) ELISA Kit is a vital tool for researchers studying immune responses and cytokine signaling. Its specificity, long shelf life, and precision make it ideal for enumerating IL-1β-secreting cells in various experimental setups.