Thermo Scientific™ Oxoid™ Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated), 500g

The Thermo Scientific™ Oxoid™ Dichloran-Glycerol 18% (DG18) (ISO) Agar Base is a selective, low water activity microbiological culture medium specifically formulated for the isolation and enumeration of yeasts and molds associated with food spoilage, particularly in dried and low-moisture food products.

This medium is fully compliant with BS ISO 21527-2:2008, the internationally recognized standard for microbial enumeration of yeasts and molds in foods with water activity ≤0.95. Its selective formulation improves fungal recovery while preventing overgrowth, allowing accurate colony counting and reliable microbiological quality control.

Key Features

• Selective culture medium for enumeration of yeasts and molds
• Fully compliant with BS ISO 21527-2:2008 microbiological standards
• Low water activity formulation enhances recovery of xerophilic molds
• Contains dichloran to restrict colony spreading and improve isolation
• Supports accurate colony counting and fungal identification
• Suitable for food microbiology quality control laboratories
• Dehydrated powder format for convenient preparation

Principle of Operation

DG18 (ISO) Agar Base contains two key functional components:

• Dichloran, which inhibits rapid spreading of mucoraceous fungi and restricts colony size, improving enumeration accuracy
• Glycerol (18% w/w), which reduces water activity (aW) to approximately 0.95, promoting selective growth of xerophilic and xerotolerant fungi

This formulation ensures reliable recovery and differentiation of molds and yeasts commonly found in dried and low-moisture food products.

Standards Compliance

This medium is formulated according to:

• BS ISO 21527-2:2008
• Microbiology of food and animal feeding stuffs
• Colony counting technique for products with water activity ≤0.95

This ensures compliance with internationally recognized microbiological testing protocols.

Applications

DG18 (ISO) Agar Base is ideal for:

• Enumeration of yeasts and molds in food samples
• Quality control testing of dried and low-moisture foods
• Food safety and spoilage investigations
• Environmental and industrial microbiology testing

Common sample types include:

• Spices
• Dried fruits
• Cereals and grains
• Nuts
• Confectionery products
• Dried meat and fish

Technical Specifications

Advantages

• ISO-compliant for food microbiology testing
• Improved recovery of xerophilic molds compared to high water activity media
• Prevents colony spreading for easier enumeration
• Ideal for low-moisture food microbiology
• Reliable and reproducible fungal detection

The Thermo Scientific™ Oxoid™ DG18 (ISO) Agar Base (CM1150B) is a selective, ISO-compliant culture medium designed for accurate enumeration and isolation of yeasts and molds in dried and low-moisture food products. Its low water activity formulation and dichloran selectivity make it an essential medium for food safety laboratories, quality control programs, and microbiological research.