0
Thermo Scientific™ Oxoid™ Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated)
Catalog No :
CAS Number :
Brand :
In Stock
Isolate and enumerate coliforms in food, animal feed, and environmental samples. Conforms to ISO 4832:2006 and ISO/TS 11133-2:2003
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated) is a selective medium designed for the isolation and enumeration of coliforms in food, animal feed, and environmental samples. This medium complies with ISO 4832:2006 and ISO/TS 11133-2:2003 standards, ensuring reliable results in microbiological analysis. By targeting lactose-fermenting organisms, VRBL Agar provides critical insights into food safety, quality, and spoilage potential.
Key Features
- Selective for Coliforms:
- Combines neutral red, crystal violet, and bile salts to inhibit Gram-positive bacteria while promoting coliform growth.
- Easy-to-Interpret Results:
- Lactose fermenters: Produce red-purple colonies with red-purple halos.
- Late lactose fermenters: Form straw or colorless colonies with greenish halos.
- Standards Compliant:
- Adheres to ISO 4832:2006 and ISO/TS 11133-2:2003 standards for food, animal feed, and environmental testing.
- Enhanced Selectivity:
- Improved differentiation by incubation at >42°C or under anaerobic conditions.
Specifications
| Property | Details |
|---|---|
| Description | Violet Red Bile Lactose Agar (VRBL) (ISO) |
| Form | Powder |
| Product Type | Agar |
| Quantity | 500 g |
| Shelf Life | 5 Years |
| Storage Conditions | 10°C to 30°C |
| Catalog Number | CM0968B |
Applications
- Food Microbiology:
- Detect and enumerate coliforms in raw and processed food products.
- Animal Feed Testing:
- Evaluate microbial contamination in feed samples.
- Environmental Monitoring:
- Monitor hygiene and microbial contamination in food production and handling facilities.
- Process Validation:
- Validate heat treatment or other contamination control measures in food processing.
Preparation Instructions
- Suspend 41.5g of VRBL Agar powder in 1 liter of distilled water.
- Heat gently to dissolve completely.
- Bring to a boil while stirring to prevent localized overheating.
- Do not autoclave. Cool to 45-50°C, pour into sterile Petri dishes, and allow to solidify.
Advantages
- Highly Selective: Suppresses Gram-positive bacteria, ensuring specificity for coliforms.
- Accurate Results: Reliable differentiation of lactose fermenters through distinct color changes.
- Standards-Compliant: Meets ISO guidelines for performance and consistency.
- Versatile Applications: Suitable for food, animal feed, and environmental monitoring.
Packaging
- Quantity: 500g of dehydrated powder.
- Yield: Prepares 12.0L of medium.
Why Choose VRBL Agar?
- A trusted medium for coliform detection and enumeration.
- Recognized for its reliability in food and environmental microbiology.
- Backed by Thermo Scientific’s commitment to quality and innovation.