Thermo Scientific™ Oxoid™ MRS Agar (DeMan, Rogosa and Sharpe) (Dehydrated)

The Thermo Scientific™ Oxoid™ MRS Broth (Dehydrated) is a non-selective medium specifically formulated for the isolation and enumeration of lactic acid bacteria from food samples. Based on the formulation developed by de Man, Rogosa, and Sharpe, MRS Broth supports robust growth of mesophilic lactic acid bacteria, making it an essential tool for food microbiology and research.

Key Features

1. Non-Selective Medium:
   • Promotes profuse growth of lactic acid bacteria, including Lactobacillus, Streptococcus, Leuconostoc, and Pediococcus species.
2. Food Microbiology Applications:
   • Ideal for isolating and enumerating lactic acid bacteria from food products.
3. Versatile Testing:
   • Supports additional identification tests such as temperature dependence, NaCl tolerance, and Teepol resistance.
4. Reproducible Results:
   • Consistently supports growth across various lactic acid bacterial strains, including slow-growing species.
5. Validated by Industry Standards:
   • Complies with guidelines from AFNOR, IDF, and NMKL.

Applications

1. Food Microbiology

• Isolation and enumeration of lactic acid bacteria from:
  • Yogurt
  • Fermented foods
  • Dairy products
  • Beverages

2. Clinical and Research Microbiology

• Growth and metabolic studies of probiotic bacteria.
• Identification and differentiation of lactobacilli.

3. Quality Control

• Microbiological testing of fermented products to ensure safety and consistency.

Specifications

Preparation Instructions

1. Suspend: Suspend 52g of MRS Broth powder in 1 liter of distilled water.
2. Mix and Heat: Heat gently with frequent agitation until fully dissolved.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Dispense: Dispense into sterile tubes, bottles, or flasks as required.

Advantages

1. Non-Selective Growth: Supports a broad range of lactic acid bacteria, including fastidious strains.
2. Versatile Testing: Facilitates identification and differentiation of lactic acid bacteria.
3. Reliable and Reproducible: Provides consistent results for food microbiology workflows.
4. Cost-Effective: High yield of 9.6L per 500g, ideal for routine laboratory use.

Quality Control

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to the product label for expiration date.

Packaging

• Product: MRS Broth (Dehydrated)
• Catalog Number: CM0359B
• Quantity: 500g
• Yield: 9.6L of prepared medium

The Thermo Scientific™ Oxoid™ MRS Broth (Dehydrated) is a reliable medium for isolating and enumerating lactic acid bacteria in food samples. Its rich composition supports consistent growth across various bacterial strains, including slow-growing species, making it a valuable tool for food safety, clinical microbiology, and research applications.