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Thermo Scientific™ Oxoid™ Modified Brilliant Green Agar (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | Target Organism Class | Escherichia Coli, Proteus species |
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Modified Brilliant Green Agar (Dehydrated) is a selective medium designed for the detection, isolation, and identification of Salmonella species (excluding Salmonella Typhi) from food, feed, and water samples. The modified formula improves inhibition of non-target organisms like Escherichia coli, Proteus species, and Pseudomonas species, ensuring more reliable Salmonella recovery. This medium complies with ISO 6579 standards, making it suitable for regulated microbiological workflows.
Key Features
- Selective for Salmonella:
- Enhances the isolation and identification of Salmonella species while restricting the growth of interfering organisms like Pseudomonas, E. coli, and Proteus.
- ISO Compliant:
- Meets the requirements of ISO 6579 for Salmonella detection, ensuring compatibility with international standards.
- Improved Formula:
- Provides greater inhibition of Escherichia coli and Proteus species compared to traditional formulations.
- Broad Application:
- Suitable for testing food, animal feed, and water samples for the presence of Salmonella.
- Widely Validated:
- Extensively assessed and used across Europe in microbiological testing protocols.
Applications
1. Food Microbiology
- Detection of Salmonella spp. in processed foods, raw meats, dairy products, and ready-to-eat foods.
2. Feed Testing
- Monitoring animal feed for contamination by Salmonella to ensure feed safety.
3. Water Microbiology
- Identifying Salmonella contamination in potable and non-potable water samples.
4. Regulatory and Compliance Testing
- Ensures adherence to food safety and public health standards through Salmonella detection.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Dehydrated Selective Agar |
| Form | Powder |
| Description | Modified Brilliant Green Agar |
| Applications | Food, feed, and water microbiology |
| Quantity | 500g |
| Compliance | ISO 6579 |
Preparation Instructions
- Suspend: Suspend 52g of Modified Brilliant Green Agar powder in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Cool and Dispense: Pour into sterile Petri dishes and allow to solidify.
Advantages
- Enhanced Selectivity: Inhibits interfering organisms like Pseudomonas, ensuring reliable Salmonella detection.
- Improved Inhibition: Offers better suppression of E. coli and Proteus species than traditional formulations.
- Regulatory Compliance: ISO 6579 compliance ensures compatibility with food safety testing protocols.
- Reproducible Results: Consistently delivers accurate and reliable Salmonella recovery.
- Broad Utility: Suitable for testing diverse sample types, including food, feed, and water.
Diagnostic Features
| Organism | Reaction |
|---|---|
| Salmonella spp. | Typical pink colonies |
| E. coli | Growth restricted |
| Proteus spp. | Growth inhibited |
| Pseudomonas spp. | Growth restricted |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to the product label for expiration date.
Packaging
- Product: Modified Brilliant Green Agar (Dehydrated)
- Quantity: 500g
The Thermo Scientific™ Oxoid™ Modified Brilliant Green Agar (Dehydrated) is a trusted and efficient medium for isolating and identifying Salmonella species from diverse sample types. Its enhanced selectivity, ISO compliance, and ease of preparation make it indispensable for laboratories focused on food safety, feed testing, and water microbiology.