Thermo Scientific™ Oxoid™ Thioglycollate Medium USP (Dehydrated)

Thioglycollate Medium USP is a microbiological medium designed for cultivating both aerobic and anaerobic organisms. It is prepared according to the formula specified in the US Pharmacopoeia (USP) and adheres to the British, European, and Japanese Pharmacopoeias, making it suitable for sterility testing and microbiological quality control.

Key Features:

1. Broad Application:
   • Suitable for cultivating aerobic and anaerobic organisms.
   • Effective for sterility testing, especially for injectable solutions and other products with potential bacteriostatic substances.
2. Well-Buffered:
   • Resistant to pH changes caused by acidic or alkaline reactions, maintaining the medium's effectiveness.
3. Neutralizing Agent:
   • Contains sodium thioglycollate, which acts as a reducing agent to neutralize the toxic effects of mercurial preservatives in solutions.
4. Supports a Range of Organisms:
   • Recommended for cultivating Clostridium species and other anaerobes.
   • Sealey’s research highlighted its effectiveness in cultivating Desulfotomaculum nigrificans.
5. Regulatory Compliance:
   • Conforms to USP, BP, EP, JP, and ISO 11133:2014 standards for sterility testing and cultivation.

Preparation Instructions:

1. Dissolve 29.6 g of medium in 1 liter of distilled water.
2. Bring the solution to a boil to dissolve completely.
3. Sterilize by autoclaving at 121°C for 15 minutes.
4. Cool and store the medium at 2-8°C until ready for use.

Composition (Typical Formula):

Microbiological Quality Control:

Appearance:

• Dehydrated Medium: Straw-colored, free-flowing powder.
• Prepared Medium: Light straw-colored gel with slight viscosity.

Storage and Shelf Life:

• Dehydrated Medium: Store at 10-30°C in a tightly sealed container.
• Prepared Medium: Store at 2-8°C, away from light and contaminants.
• Shelf Life: 5 years for the dehydrated medium.

Applications:

1. Sterility testing for products, especially solutions containing bacteriostatic substances.
2. Cultivation of anaerobic and facultative organisms, including Clostridium species.
3. Monitoring microbial quality in pharmaceuticals and other sensitive industries.

Packaging Options:

• Catalog Number CM0173B: 500 g, yielding 16.9 liters of prepared medium.
• Catalog Number CM0173T: 5 kg for larger-scale applications.

Regulatory Compliance:

• Meets USP, BP, EP, JP, and ISO 11133:2014 standards for sterility testing and cultivation.

This product is a reliable choice for laboratories conducting sterility testing, supporting aerobic and anaerobic cultivation, and maintaining compliance with international quality standards.

Thermo Scientific™ Oxoid™ USP Alternative Thioglycolate Broth (CM0391B) vs. Thioglycollate Medium USP (CM0173B)

This table highlights the differences and similarities between the two Thioglycollate media types, helping users select the appropriate medium based on their specific application needs.