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Thermo Scientific™ 10X Buffer Set for Restriction Enzymes, 5 x 1.0 mL
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Specifications:
| Application | Cloning, Transfection | ||
| Storage Temperature | -20°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Five Buffer System is designed to provide optimal reaction conditions for Thermo Scientific restriction enzymes. Each buffer is color-coded—Blue (B), Green (G), Orange (O), Red (R), and Yellow (Tango)—for easy identification and consistent performance across a wide variety of restriction digests. These 10X concentrated buffers ensure 100% enzyme activity when used as recommended.
Tango Buffer is uniquely formulated for double digestions and serves as a universal option for many restriction enzymes. All buffers include BSA to enhance enzyme stability and performance, even after multiple freeze-thaw cycles.
Key Features
- Complete Set – Contains 1 mL each of Buffers B, G, O, R, and Tango
- Color-Coded Tubes – Matches enzyme labeling for quick buffer identification
- 10X Concentrated – Convenient format for dilution and storage
- BSA-Enhanced Stability – Improves enzyme activity and protects against contaminants
- Supports Double Digestions – Tango buffer is optimized for two-enzyme reactions
Specifications
| Property | Value |
|---|---|
| Product Name | Buffer Set for Restriction Enzymes |
| Catalog Number | B30 |
| Quantity | 5 × 1.0 mL |
| Concentration | 10X stock solutions |
| Color Codes | B (Blue), G (Green), O (Orange), R (Red), Tango (Yellow) |
| BSA Included | Yes |
| Application | Restriction digestion, double digestion |
| Packaging | Vials, 1 mL each |
| Storage Conditions | –20 °C recommended |
| Research Category | Traditional Cloning |
| Use Restriction | For research use only |
Applications
- Restriction Enzyme Digestion – Provides optimal buffer conditions for over 150 enzymes
- Double Digestions – Tango buffer simplifies multi-enzyme protocols
- Molecular Cloning – Essential component of cloning workflows
- Gene Editing & DNA Mapping – For accurate and efficient DNA cleavage
- Teaching & Research Labs – For standard molecular biology procedures
Ordering Information
| Catalog Number | Description | Pack Size |
|---|---|---|
| B30 | Five Buffer Set (10X) | 5 × 1.0 mL |
Thermo Scientific™ Buffer Set for Restriction Enzymes provides a reliable, user-friendly solution for ensuring optimal enzyme performance across a wide range of molecular biology protocols. The inclusion of BSA and universal Tango buffer enhances utility in both standard and complex digestion workflows.