Appolo Sci. 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside

Fluorogenic Substrate for β-N-acetylglucosaminidase, Chitinase, and N-acetylhexosaminidase Enzyme Assays

Apollo Scientific™ 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside (commonly referred to as 4-MUF-NAG) is a synthetic, fluorogenic substrate designed for the quantitative measurement of N-acetyl-β-D-glucosaminidase (NAGase) and related enzymes. It consists of a chitin monomer unit (N-acetyl-D-glucosamine, NAG) linked to a fluorescent 4-methylumbelliferone (4-MUF) group.

Upon enzymatic cleavage by NAGase, chitinase, or β-N-acetylhexosaminidase, the fluorescent 4-MUF moiety is released, allowing rapid, sensitive detection through fluorimetry. This reagent is highly valued in plant biology, soil microbiology, enzymology, and fungal studies.

Key Features & Benefits

• Fluorogenic Detection – Enables real-time, high-sensitivity enzyme activity assays
• Specific Substrate – Targets β-N-acetylglucosaminidase (NAGase), chitinase, and β-N-acetylhexosaminidase
• Versatile Use – Compatible with assays in soil, plant tissues, microbial cultures, and enzymatic extracts
• Coumarin-Based Fluorophore – Strong fluorescence at ~445 nm upon 4-MUF release
• Trusted for Research – Widely used in enzymatic assays for plant pathology, microbial ecology, and biochemistry

Applications

• β-N-acetylglucosaminidase (NAGase) Assays – From soil, fungal, bacterial, or animal extracts
• Chitinase Activity Measurement – In fungal studies (e.g., Fusarium oxysporum) and biocontrol screening
• β-N-acetylhexosaminidase Assays – In plant models such as Arabidopsis thaliana
• Fluorescence-Based Enzymology – Used in kinetic, inhibition, and substrate-specificity studies
• Environmental & Agricultural Research – Monitoring of microbial enzyme activity in soil health evaluations

Biochemical/Physiological Role

• Synthetic analog of natural chitin degradation products
• Substrate specificity: cleaved by NAGase, chitinase, and hexosaminidase enzymes
• Fluorescence signal correlates directly with enzymatic hydrolysis activity
• Utilized in understanding plant immunity, soil enzyme dynamics, and pathogen resistance mechanisms

Specifications

Apollo Scientific™ 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside (4-MUF-NAG) is a highly sensitive fluorogenic substrate tailored for assaying β-N-acetylglucosaminidase, chitinase, and hexosaminidase enzymes across a broad range of biological systems. Its compatibility with fluorescence-based detection methods makes it an indispensable tool in enzymology, microbiology, and plant science research.