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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR
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Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C ±5°C in the dark | ||
| Product Type | Real Time PCR SYBR Master Mix | Forms | Freeze Dried |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
PowerTrack™ SYBR Green Master Mix is an optimized, ready-to-use 2X master mix for quantitative real-time PCR (qPCR) that delivers exceptional specificity, reproducibility, and visual confidence during setup. Designed to work across a broad range of primer sets and thermocyclers, it provides reliable amplification of DNA targets for applications such as gene expression analysis and virus detection.
With built-in visual indicators, optional yellow sample buffer, and UNG/dUTP system to prevent carryover contamination, PowerTrack Master Mix is an ideal choice for high-performance and high-throughput qPCR workflows.
Key Features
🎯 Optimized Performance & Versatility
- Broad compatibility with primer Tm and concentration ranges (300–800 nM)
- Works with standard or fast qPCR protocols
- Compatible with Applied Biosystems, Bio-Rad, Roche, and Agilent qPCR platforms
🧪 Built-In Visual Setup Verification
- Includes an inert blue dye in the master mix for better visibility during pipetting
- Optional yellow sample buffer causes a color shift from blue to green when added—providing visual confirmation of correct reaction setup
🔬 High Specificity and Sensitivity
- Hot-start Taq DNA Polymerase controlled by an antibody-mediated mechanism
- Minimizes non-specific amplification and primer-dimer formation
🔒 Carryover Contamination Control
- Formulated with UNG and dUTP to eliminate previously amplified products
- Reduces risk of false positives in high-throughput or clinical research labs
📈 Reliable Results Across Applications
- Linear amplification across a broad dynamic range
-
Suitable for:
- Gene expression analysis
- Viral load quantification
- Pathogen detection
- General qPCR workflows
Instrument Compatibility
PowerTrack SYBR Green Master Mix is validated for use with:
- Applied Biosystems™: 7500, 7900HT, QuantStudio 3/5/6/7/12K Flex, ViiA™ 7, StepOne™, StepOnePlus™
- Bio-Rad™: CFX96™, CFX384™, iQ™, iCycler™, MyiQ™
- Roche™: LightCycler™ 480
- Agilent/Stratagene™: Mx3000P™, Mx3005P™, Mx4000™
- MJ Research™: Opticon™, Chromo4™
Specifications
| Attribute | Details |
|---|---|
| Detection Method | SYBR Green I |
| Format | Tube |
| Polymerase | Antibody-mediated Hot-start Taq DNA Polymerase |
| Concentration | 2X |
| Passive Reference Dye | ROX (pre-mixed) |
| Reaction Speed | Standard or Fast |
| PCR Method | qPCR |
| Final Product Type | Amplified DNA for Quantification |
| Shipping Condition | Dry Ice |
| Storage Conditions | –20°C ±5°C, protected from light |
| Applications | Gene Expression, Virus Detection |
| Sample Type | DNA |
| Volume Sizes Available | 1 mL, 5 mL, 10 mL, 25 mL, 50 mL |
| Includes | PowerTrack SYBR Green Master Mix, 40X Yellow Sample Buffer |
Ordering Information
| Catalog Number | Volume | No. of Reactions (Approx.) |
|---|---|---|
| A46012 | 1 mL | ~100 reactions (20 µL each) |
| A46109 | 5 mL | ~500 reactions |
| A46110 | 2 × 5 mL | ~1,000 reactions |
| A46111 | 5 × 5 mL | ~2,500 reactions |
| A46112 | 10 × 5 mL | ~5,000 reactions |
| A46113 | 50 mL | ~5,000 reactions |
Reaction numbers based on standard 20 µL format.
✅ Comparison: PowerUp™ vs. PowerTrack™ SYBR Green Master Mix
| Feature | PowerUp™ SYBR Green Master Mix | PowerTrack™ SYBR Green Master Mix |
|---|---|---|
| Enzyme System | Dual-Lock™ hot-start Taq (antibody + chemical) | Antibody-mediated hot-start Taq |
| Visual Dye Indicators | ❌ None | ✅ Blue master mix + optional yellow buffer (turns green when combined) |
| Carryover Contamination Control | ✅ Includes UDG/dUTP | ✅ Includes UNG/dUTP |
| Pre-Assembled Reaction Stability | ✅ Up to 72 hours at room temperature before cycling | ❌ Not specified |
| Reproducibility | ✅ Tight CTs, 6-log dynamic range | ✅ Consistent CTs across a broad dynamic range |
| Optimization Requirements | Low (broad primer Tm and concentration compatibility) | Minimal optimization needed |
| Visual Setup Confirmation | ❌ Not available | ✅ Color change ensures pipetting accuracy |
| Instrument Compatibility | Applied Biosystems™, Bio-Rad™, Roche™, Agilent™ | Applied Biosystems™, Bio-Rad™, Roche™, Agilent™ |
| Best For | ✅ High-throughput, automation, gene expression, contamination-sensitive workflows | ✅ Beginner-friendly, visual confirmation, educational labs, qPCR newbies |
Recommendations by Use Case
| Application | Best Choice | Why |
|---|---|---|
| Routine gene expression (qPCR) | PowerUp™ | Higher reproducibility, batch setup stability |
| Educational/teaching labs | PowerTrack™ | Visual pipetting confirmation (color change) |
| Carryover contamination-prone labs | Both | Both contain UNG/UDG and dUTP |
| First-time qPCR users | PowerTrack™ | Easy setup with color-coded reagents |
| High-throughput labs (automation) | PowerUp™ | Stable pre-assembled reactions for up to 72 hours |
| Low-expressing gene detection | PowerUp™ | Superior enzyme control with dual hot-start system |
- Pack Size: 1 x 1 mL, sufficient for 100 Rxns 1 x 5 mL, sufficient for 500 Rxns 2 x 5 mL, sufficient for 1000 Rxns 5 x 5 mL, sufficient for 2500 Rxns 10 x 5 mL, sufficient for 5000 Rxns 1 x 50 mL, sufficient for 5000 Rxns
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