Apollo Sci. MBS (N-Succinimidyl 3-maleimidobenzoate)

Apollo Scientific MBS (N-Succinimidyl 3-maleimidobenzoate) is a heterobifunctional crosslinker designed for bioconjugation and protein labeling. It contains NHS-ester (N-hydroxysuccinimide ester) and maleimide reactive groups, allowing for selective and efficient conjugation of amines (-NH₂) and sulfhydryls (-SH) in biomolecules such as proteins, peptides, and antibodies.

Key Features & Benefits:

• Heterobifunctional Crosslinker – Contains both amine-reactive NHS ester and thiol-reactive maleimide groups.
• Highly Selective Conjugation – Efficiently links amine-containing biomolecules (e.g., lysine residues in proteins) with thiol-containing biomolecules (e.g., cysteine residues in peptides or proteins).
• Stable, Non-Cleavable Bonding – Forms a permanent, stable amide-thioether linkage, ensuring strong and irreversible conjugation.
• Ideal for Protein Labeling & Hapten Carrier Applications – Suitable for ELISA, Western blotting, immunoassays, and drug delivery studies.
• Water-Insoluble – Requires DMSO or DMF for dissolution before use.

Applications:

1. Bioconjugation & Protein Labeling

• Conjugation of enzymes, antibodies, and proteins for use in ELISA, Western blotting, and immunohistochemistry.
• Labeling fluorophores, haptens, and nanoparticles to proteins for biosensing and imaging studies.

2. Antibody-Drug Conjugates (ADCs) & Drug Development

• Functionalization of antibodies and therapeutic biomolecules for targeted drug delivery.
• Conjugation of small molecules and drugs to carrier proteins for vaccine development.

3. Nanoparticle & Surface Modification

• Covalent attachment of gold nanoparticles, quantum dots, and magnetic beads to biomolecules.
• Functionalization of biosensor surfaces for detection of biomolecular interactions.

4. Hapten Carrier Conjugation

• Conjugation of small molecules (haptens) to carrier proteins such as KLH, BSA, and OVA for antibody production.

5. Molecular Biology & Synthetic Biology

• Site-specific modification of DNA, RNA, or peptides for molecular probes and biosensors.
• Crosslinking of cell surface receptors for studying cell signaling pathways.

Specifications:

Handling & Storage:

• Storage: Keep at -20°C in a desiccated environment to maintain stability.
• Solubility: Dissolve in anhydrous DMSO or DMF before adding to aqueous solutions.
• Reactivity Considerations: Avoid exposure to moisture and store under inert gas to prevent hydrolysis.

Comparison with Other Crosslinkers:

Related Products:

• Apollo Scientific Sulfo-MBS – A water-soluble alternative for aqueous conjugation.
• Apollo Scientific SMCC (Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) – A similar heterobifunctional crosslinker with a rigid cyclohexane spacer.
• Apollo Scientific BS³ (Bis-sulfosuccinimidyl suberate) – Amine-to-amine crosslinker for protein conjugation.

Pack Sizes & Ordering Information:

Apollo Scientific MBS (N-Succinimidyl 3-maleimidobenzoate) is a powerful bioconjugation reagent with broad applications in protein labeling, antibody-drug conjugates (ADCs), and nanoparticle functionalization. Its high specificity, stability, and strong covalent bonding make it an ideal choice for biosensor development, molecular imaging, and therapeutic research.