Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 96 reactions, 0.1 mL

Multiplex real-time PCR kit for simultaneous detection of Zika, Dengue, and Chikungunya virus RNA in urine and serum

The Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit is a lyophilized, multiplex, one-step real-time PCR assay designed for the simultaneous detection and differentiation of three clinically important arboviruses—Zika virus (ZIKV), Dengue virus (DENV1–4), and Chikungunya virus (CHIKV)—directly from human urine or serum samples.

Optimized for use with the Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System, this kit is also compatible with the 7500, 7500 Fast, and other QuantStudio systems. It includes a human PPIA (cyclophilin A) primer-probe set to serve as an endogenous extraction and amplification control, ensuring the integrity and reliability of assay results.

The assay’s lyophilized format eliminates the need for cold chain management, enabling room temperature shipping and storage, making it ideal for field use and resource-limited settings.

Key Features & Benefits

• Triple Target Detection – Detect and differentiate ZIKV, DENV (all 4 serotypes), and CHIKV in a single reaction.
• Lyophilized One-Step RT-PCR – Master mix and primers are pre-formulated; simply add RNA.
• Endogenous Control – Human PPIA ensures proper extraction and RT-PCR performance monitoring.
• Environmentally Friendly & Convenient – No refrigeration required; stable at room temperature.
• Broad Instrument Compatibility – Validated for QuantStudio™ 5, 7500, 7500 Fast, and other QuantStudio systems.
• Rapid & Reliable – Supports both fast and standard cycling protocols for flexible workflows.

Applications

• Multiplex detection of arboviruses in human clinical samples
• Public health surveillance and outbreak monitoring
• Arbovirus diagnostic research and assay validation
• Laboratory preparedness for vector-borne disease detection

Technical Specifications

Contents

• 12 × 8-well strip tubes (96 total reactions)
• Primer-probe mixes for ZIKV, DENV, CHIKV, and human PPIA
• Lyophilized one-step RT-PCR master mix

The Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.1 mL offers an efficient, multiplexed, and field-ready solution for detecting three major arboviruses in human urine and serum. With its lyophilized one-step RT-PCR format, built-in endogenous control, and room-temperature stability, it streamlines laboratory workflows while maintaining high sensitivity and specificity. This makes it an invaluable tool for clinical research, epidemiological surveillance, and laboratory readiness against vector-borne viral threats.