Liofilchem® Ofloxacin OFX, Antimicrobial Susceptibility Discs, 5 µg

Liofilchem® Ofloxacin OFX Antimicrobial Susceptibility Discs (5 µg) are high-performance, ready-to-use paper discs for in vitro determination of bacterial susceptibility to ofloxacin using the Kirby-Bauer disk diffusion method. Each disc is impregnated with 5 micrograms of ofloxacin, a broad-spectrum fluoroquinolone antibiotic effective against a wide range of Gram-negative and Gram-positive bacteria.

These discs are manufactured under ISO 9001 and ISO 13485 standards, ensuring sterility, consistency, and traceability for use in clinical, pharmaceutical, veterinary, and research microbiology laboratories.

Key Features

• Accurate potency: Contains precisely 5 µg of ofloxacin per disc for standardized antimicrobial susceptibility testing (AST).
• Broad-spectrum activity: Effective against Escherichia coli, Salmonella spp., Shigella spp., Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Haemophilus influenzae.
• CLSI/EUCAST compliant: Suitable for susceptibility interpretation using international reference standards.
• Stable and sterile: Supplied in desiccated, sealed cartridges or canisters to maintain potency and prevent degradation.
• Color-coded labeling: Enhances lab workflow and traceability during high-volume susceptibility testing.

Applications and Use Cases

1. Clinical Microbiology

• Susceptibility testing of pathogens involved in urinary tract infections (UTIs), respiratory tract infections, skin and soft tissue infections, and gastrointestinal infections.
• Detection of fluoroquinolone resistance in Gram-negative bacilli and Gram-positive cocci.

2. Veterinary Microbiology

• AST for E. coli, Pasteurella, and Staphylococcus species in livestock and companion animals.

3. Public Health Surveillance

• Resistance monitoring for foodborne pathogens such as Salmonella and Shigella in environmental and clinical settings.

4. Pharmaceutical and Research Laboratories

• Evaluation of new fluoroquinolone formulations.
• MIC correlation studies with fluoroquinolones for pharmacodynamic analysis.

Use Instructions

1. Medium: Mueller-Hinton Agar (MHA) is recommended.
2. Inoculum preparation: Adjust the bacterial suspension to 0.5 McFarland turbidity standard.
3. Inoculation: Spread the inoculum uniformly over the agar surface using a sterile swab.
4. Disc placement: Place one 5 µg Ofloxacin disc on the agar using sterile forceps or a disc dispenser.
5. Incubation: 35 ± 2 °C for 16–18 hours under aerobic conditions.
6. Result interpretation: Measure the diameter of the inhibition zone (mm) and interpret using CLSI or EUCAST zone diameter breakpoints.

Technical Specifications

Quality Control

Each batch is tested using standard reference strains under CLSI/EUCAST protocols:

Liofilchem® Ofloxacin OFX Discs (5 µg) offer a reliable, standardized solution for antimicrobial susceptibility testing of one of the most widely used fluoroquinolones. Their broad-spectrum coverage, quality-assured manufacturing, and compatibility with global AST guidelines make them indispensable in microbiology diagnostics, resistance surveillance, and pharmaceutical testing environments.