Liofilchem® Minocycline MN, Antimicrobial Susceptibility Discs, 30 µg

The Liofilchem® Minocycline (MN) Antimicrobial Susceptibility Discs, 30 µg are standardized diagnostic tools for evaluating bacterial susceptibility to minocycline, a broad-spectrum tetracycline antibiotic. These discs play a vital role in antimicrobial susceptibility testing (AST), providing reliable and reproducible results to guide antibiotic therapy and monitor resistance trends.

Key Features:

1. Standardized Dosage:
   • Each disc is impregnated with 30 µg of minocycline, ensuring consistency in testing and accurate results.
2. High Quality:
   • Manufactured under stringent quality controls to meet international standards (e.g., CLSI and EUCAST).
3. Pre-Packaged and Ready-to-Use:
   • Supplied in sterile cartridges for ease of handling and minimized risk of contamination.
4. Broad Spectrum of Activity:
   • Effective against a wide range of gram-positive and gram-negative bacteria, including Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Acinetobacter baumannii.
5. Reliable Performance:
   • Designed for consistent and reproducible results across diverse bacterial species and laboratory settings.

Applications:

1. Clinical Diagnostics:
   • Determines bacterial susceptibility to minocycline, helping clinicians select appropriate antibiotic therapy.
2. Research and Development:
   • Supports studies on bacterial resistance mechanisms and minocycline efficacy.
3. Public Health Surveillance:
   • Monitors antimicrobial resistance patterns, contributing to infection control strategies.
4. Pharmaceutical Quality Control:
   • Ensures the effectiveness of minocycline formulations in clinical microbiology settings.

Use Cases:

1. Respiratory Tract Infections:
   • Assesses minocycline activity against pathogens such as Haemophilus influenzae and Streptococcus pneumoniae.
2. Skin and Soft Tissue Infections:
   • Tests susceptibility of Staphylococcus aureus (including MRSA) to minocycline.
3. Urinary Tract Infections (UTIs):
   • Evaluates activity against uropathogens like Escherichia coli and Enterococcus faecalis.
4. Multidrug-Resistant Infections:
   • Tests efficacy against resistant strains such as Acinetobacter baumannii and Klebsiella pneumoniae.

Specifications:

Testing Procedure:

1. Preparation:
   • Inoculate a Mueller-Hinton agar plate with a standardized bacterial suspension (e.g., 0.5 McFarland standard).
2. Disc Application:
   • Place the minocycline disc onto the agar surface using sterile forceps or a disc dispenser.
3. Incubation:
   • Incubate the plate at 35°C for 16–18 hours in an aerobic atmosphere.
4. Result Interpretation:
   • Measure the diameter of the zone of inhibition around the disc.
   • Compare results to CLSI/EUCAST interpretive criteria to classify the organism as susceptible (S), intermediate (I), or resistant (R).

Advantages:

1. Accurate and Reliable:
   • Provides precise and reproducible susceptibility data to guide antibiotic selection.
2. Ease of Use:
   • Pre-packaged discs simplify handling and reduce contamination risks.
3. Broad Applicability:
   • Effective against diverse pathogens, making it suitable for clinical, research, and industrial microbiology.
4. Supports Resistance Monitoring:
   • Essential for tracking resistance trends in bacterial pathogens.

Storage and Handling:

• Store at 2°C to 8°C in a dry environment, away from light and moisture.
• Use discs before the expiration date indicated on the label.
• Avoid prolonged exposure to room temperature to maintain potency.

Product Details:

Pathogens Typically Tested:

• Escherichia coli
• Staphylococcus aureus (including MRSA)
• Klebsiella pneumoniae
• Acinetobacter baumannii
• Haemophilus influenzae
• Pseudomonas aeruginosa

Quality Control:

• Control Strains:
  • Escherichia coli ATCC® 25922 (positive control, susceptible).
  • Staphylococcus aureus ATCC® 25923 (positive control, susceptible).
  • Pseudomonas aeruginosa ATCC® 27853 (negative control, resistant).
• Regular quality control testing ensures the reliability of results and compliance with international standards.

The Liofilchem® Minocycline (MN) Antimicrobial Susceptibility Discs, 30 µg are indispensable for clinical diagnostics, research, and antimicrobial resistance monitoring. Their high quality, broad-spectrum activity, and ease of use make them a trusted tool in microbiology laboratories worldwide.