Thermo Scientific™ ActivX™ Azido-FP Serine Hydrolase Probe, 3.5 μg

Thermo Scientific™ ActivX™ Azido-FP Serine Hydrolase Probe is an activity-based fluorophosphonate probe designed for selective labeling of enzymatically active serine hydrolases. The probe contains a reactive fluorophosphonate (FP) group that covalently labels the active-site serine residue of functional serine hydrolases, together with an azido group for downstream detection or enrichment using phosphine- or alkyne-derivatized tags.

This probe is useful for profiling serine hydrolase activity in cells, lysates, tissues, subcellular fractions, and recombinant protein preparations. It supports workflows such as activity-based protein profiling, pull-down enrichment, Western blotting, chemiluminescent detection, fluorescent tagging, and LC-MS/MS-based proteomics.

Key Features

Applications

ActivX™ Azido-FP Serine Hydrolase Probe is suitable for:

• Serine hydrolase activity profiling
• Detection of active serine hydrolases in cells and lysates
• Mapping active-site serine residues
• Profiling proteases, lipases, esterases, amidases, and peptidases
• Screening small molecule binding affinities
• Active-site inhibition studies
• Inhibitor target and off-target profiling
• Pull-down enrichment workflows
• Western blot-based enzyme activity studies
• LC-MS/MS-based activity profiling
• Click chemistry-based detection or enrichment workflows

Product Specifications

Workflow Overview

Ordering Information

Storage & Handling

Store the probe at -80 °C, protected from light. Stock solutions prepared in DMSO can be stored at -20 °C for up to 6 months. Handle using appropriate laboratory personal protective equipment and avoid repeated freeze-thaw cycles where possible.

Research Use Statement

For Research Use Only (RUO). Not for use in diagnostic procedures. This product is subject to a limited use label license.