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Invitrogen™ Denhardt's Solution (50X), 100mL
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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Sterile Filtered Blocking Reagent for Hybridization Protocols
Denhardt’s Solution (50X) is a ready-to-use blocking reagent widely used in membrane-based nucleic acid hybridization techniques such as Northern and Southern blots. This solution consists of a standardized mix of three blocking agents—1% Ficoll 400, 1% polyvinylpyrrolidone (PVP), and 1% bovine serum albumin (BSA)—which reduce nonspecific binding of nucleic acid probes to the membrane, thereby increasing hybridization signal clarity and specificity.
This UltraPure™-grade reagent is sterile-filtered, ensuring high quality and low background for sensitive applications. It is supplied as a 50X concentrate, allowing flexible dilution based on experimental requirements.
Key Features & Benefits
✅ 50X Concentrated Format – Convenient, economical dilution for multiple hybridizations
✅ Reduces Nonspecific Binding – Improves clarity and specificity of hybridization signals
✅ Sterile-Filtered, Ready to Use – Ensures reagent purity for sensitive detection systems
✅ UltraPure™ Grade – Ideal for high-performance molecular biology protocols
✅ Long-Term Storage – Stable at -5 to -30°C
Applications
- Southern Blotting – DNA hybridization
- Northern Blotting – RNA hybridization
- Colony and Plaque Hybridizations – Probe-based detection of clones
- Microarray Blocking Protocols
- General Nucleic Acid Probe-Based Membrane Workflows
Technical Specifications
| Attribute | Details |
|---|---|
| Product Name | Denhardt’s Solution (50X) |
| Catalog Number | 750018 |
| Product Type | Blotting Buffer / Blocking Reagent |
| Buffer Type | Hybridization Buffer Additive |
| Concentration | 50X |
| Contents | 1% Ficoll 400, 1% PVP, 1% BSA |
| Quantity | 100 mL |
| Purity Grade | UltraPure™ |
| Sterility | Sterile-filtered |
| Storage Temperature | –5 to –30°C |
| Unit Size | Each (100 mL bottle) |
| Shipping Conditions | Room Temperature |
| Research Use | For Research Use Only; Not for diagnostic use |
Storage & Handling
- Store at -5 to -30°C.
- Thaw completely and mix well before use.
- Avoid repeated freeze-thaw cycles to maintain reagent stability.
Invitrogen™ Denhardt’s Solution (50X) is a trusted hybridization blocking reagent for researchers conducting membrane-based nucleic acid detection. Its carefully optimized formulation reduces background signal, improves probe specificity, and supports reliable results across molecular biology workflows. Backed by UltraPure™ quality, it is the reagent of choice for sensitive blotting and hybridization protocols.