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Sigma-Aldrich™ Novagen® pET-15b DNA Vector
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Specifications:
| Application | Cloning | ||
| Storage Temperature | –80°C | ||
| Product Type | Plasmids | Forms | Liquid |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Molecular Biology | ||
The Sigma-Aldrich™ Novagen® pET-15b DNA Vector is a high-performance plasmid designed for efficient recombinant protein expression in E. coli using the powerful T7 promoter system. This vector features an N-terminal HIS TAG® sequence, enabling rapid purification of expressed proteins via immobilized metal affinity chromatography (IMAC), along with a thrombin cleavage site for optional removal of the affinity tag after purification.
The pET-15b vector is part of the widely trusted Novagen® pET expression system, known for delivering high-level, tightly regulated expression of recombinant proteins. Supplied as purified plasmid DNA, it also includes an induction control strain, ensuring reliable experimental setup and expression validation.
This vector is ideal for applications in molecular cloning, recombinant protein production, structural biology, enzyme studies, and biotechnology research.
Key Features and Advantages
Efficient T7 Promoter Expression System
- Strong T7 promoter enables high-level protein expression
- Tight regulation minimizes basal expression
- Ideal for expressing toxic or difficult proteins
N-terminal HIS TAG® Fusion for Easy Purification
- Built-in 6×His tag enables rapid purification using Ni-NTA or IMAC resins
- Reduces purification time and improves protein yield
- Suitable for large-scale and analytical protein purification
Thrombin Cleavage Site for Tag Removal
- Allows removal of HIS tag after purification
- Produces native protein when required
- Ideal for structural and functional studies
Multiple Cloning Sites (MCS)
- Provides flexible cloning options
- Supports insertion of various gene sequences
- Facilitates efficient molecular cloning workflows
High-Quality, Ready-to-Use Plasmid DNA
- Supplied as purified plasmid DNA (10 μg)
- Includes induction control strain for expression validation
- Ensures reliable experimental results
Applications
The Novagen® pET-15b vector is ideal for:
- Recombinant protein expression in E. coli
- His-tagged protein purification
- Structural biology and protein crystallography
- Enzyme expression and characterization
- Molecular cloning and gene expression studies
- Biotechnology and life sciences research
- Production of recombinant enzymes and proteins
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | pET-15b DNA Vector |
| Brand | Novagen® (Sigma-Aldrich™) |
| Pack Size | 10 μg |
| Catalog Number | 69661-3 |
| Expression System | T7 promoter |
| Tag | N-terminal HIS TAG® |
| Cleavage Site | Thrombin cleavage site |
| Cloning Sites | Multiple cloning sites (MCS) |
| Host Compatibility | E. coli expression systems |
| Form | Purified plasmid DNA |
| Storage Temperature | −70°C |
| Shipping Conditions | Dry ice / Wet ice |
| Storage Condition | OK to freeze, avoid repeated freeze/thaw cycles |
| Quality Level | 100 |
| Application Category | Cloning & Expression |
System Components
Each order includes:
- Purified pET-15b plasmid DNA (10 μg)
- Induction control strain (glycerol stock)
- Vector map and technical documentation
Unique Advantages
- High-level recombinant protein expression
- Built-in HIS tag simplifies purification
- Flexible cloning and expression options
- Reliable Novagen® pET system performance
- Ideal for research laboratories and protein production workflows
Regulatory and Use Information
- For research use only (RUO)
- Contains genetically modified organism (GMO) components
- Not intended for therapeutic or commercial manufacturing use without licensing
The Sigma-Aldrich™ Novagen® pET-15b DNA Vector is a powerful and reliable tool for recombinant protein expression in E. coli. With its integrated HIS TAG®, thrombin cleavage site, and strong T7 promoter system, it enables efficient protein expression, purification, and downstream analysis. This vector is an essential solution for molecular biology, biotechnology, and protein research laboratories.