Invitrogen™ eBioscience™ CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), eFluor™ 450

The Invitrogen™ CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), conjugated to eFluor™ 450, is a high-performance antibody specifically designed for flow cytometric analysis. This antibody targets CD24, a glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on B cells, myeloid lineage cells, and certain cancer cell types. With bright fluorescence emission and excellent specificity, this antibody is an essential tool for immunophenotyping, cancer research, and studying B-cell development and regulation.

Key Features:

1. Target and Specificity:
   • Recognizes CD24, also known as Heat Stable Antigen (HAS), a 35–50 kDa molecule involved in B-cell development, regulation, and cancer progression.
2. Clone and Conjugate:
   • Clone: eBioSN3 (SN3 A5-2H10), ensuring high specificity and reproducibility.
   • Conjugate: eFluor™ 450, a bright fluorochrome with excitation at 405 nm and emission at 445 nm, offering a reliable alternative to Pacific Blue®.
3. Applications:
   • Validated for flow cytometry and immunocytochemistry (ICC/IF).
   • Ideal for detecting CD24 expression on various cell types, including lymphocytes, erythrocytes, and cancer cells.
4. Pre-Titrated and Ready-to-Use:
   • Each test is pre-titrated for ease of use, requiring only 5 µL (0.25 µg) per 100 µL of sample for optimal staining.
5. High Performance:
   • Provides bright fluorescence and low background for clear and reliable flow cytometric data.

Specifications:

Applications:

1. Flow Cytometry:
   • Detects CD24 expression on granulocytes, B cells, thymocytes, and other cell types.
   • Provides high sensitivity for identifying cell populations in human peripheral blood samples.
2. Immunocytochemistry (ICC/IF):
   • Suitable for detecting CD24 localization in fixed cells or tissue samples.
3. Cancer Research:
   • Investigate the role of CD24 in tumorigenesis, particularly in breast cancer, lung cancer, and other malignancies where CD24 expression is a key marker.
4. B-Cell Research:
   • Analyze B-cell development, proliferation, and regulation of autoimmunity.

Key Benefits:

1. Bright Fluorescence:
   • eFluor™ 450 provides clear and stable signal detection, ideal for multicolor flow cytometry panels.
2. High Specificity and Sensitivity:
   • The eBioSN3 clone offers reliable detection of CD24-expressing cells with minimal background noise.
3. User-Friendly:
   • Pre-titrated and ready-to-use formulation simplifies experimental workflows.
4. Reproducibility:
   • Validated for flow cytometry and immunocytochemistry, ensuring consistent and reproducible results.
5. Versatile Research Tool:
   • Applicable in studies of immune regulation, cancer progression, and B-cell biology.

Usage Recommendations:

1. Sample Preparation:
   • Stain 10⁵–10⁸ cells per test in a final volume of 100 µL using 5 µL of antibody solution.
2. Flow Cytometry:
   • Analyze using a flow cytometer equipped with a violet laser (405 nm).
3. Fixation:
   • Use suitable fixation buffers to preserve fluorochrome integrity.

Storage and Handling:

• Store at 4°C in the dark to protect the eFluor™ 450 signal.
• Do not freeze to prevent damage to the antibody or fluorochrome.

Target Information:

• CD24 (Heat Stable Antigen):
  • A GPI-anchored glycoprotein expressed during B-cell development and on various cancers.
  • Plays a role in cell adhesion, immune response regulation, and tumorigenesis.
  • Expressed in normal granulocytes, thymocytes, peripheral lymphocytes, and erythrocytes.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.