BD™ CD4 PerCP, 100 Tests

BD™ CD4 PerCP (100 Tests)

The BD™ CD4 PerCP reagent is designed for in vitro diagnostic use in the identification of cells expressing the CD4 antigen, primarily in flow cytometry applications. This reagent leverages PerCP (Peridinin-Chlorophyll Protein), a fluorescent dye in the BD blue family, to provide high-performance fluorescence for precise cell analysis.

Key Features

1. Intended Use:
   • For in vitro diagnostics using BD FACS™ flow cytometers equipped with appropriate light scatter and fluorescence detection capabilities.
2. Fluorescent Dye (PerCP):
   • Excitation Max: 481 nm (excited by blue laser at 488 nm).
   • Emission Max: 675 nm (detected using a 695/40 nm bandpass filter).
   • Cross-laser excitation can occur with violet lasers (405 nm), resulting in spillover.
3. Reagent Details:
   • Isotype: Mouse BALB/c IgG1, κ.
   • Concentration: 3 μg/mL.
   • Volume Per Test: 20 μL.
4. Applications:
   • Designed for flow cytometry to identify human CD4-positive cells, including T-helper lymphocytes.
5. Storage Conditions:
   • Stable at 2° to 8°C.
   • Do not freeze or expose to direct light.

Specifications

Dye Properties (PerCP)

Applications

1. Flow Cytometry:
   • Detection and quantification of CD4+ T cells.
   • Analysis of immune cell populations in human peripheral blood.
2. Diagnostic Use:
   • For in vitro diagnostic purposes, including monitoring immune system health.

Preparation and Handling

• Store at 2° to 8°C; avoid freezing.
• Protect from direct light to maintain reagent stability.
• Inspect for precipitation or discoloration, which indicates reagent deterioration.
• Ensure the flow cytometer is equipped with the appropriate laser and optical filter configurations for PerCP detection.

Important Notes

• Instrument Compatibility: Ensure the BD FACS™ cytometer is configured to detect the 481 nm excitation and 675 nm emission signals.
• Volume Per Test: Use 20 μL of the reagent for each test.
• Shelf Life: Do not use past the expiration date on the label.

The BD™ CD4 PerCP reagent combines the sensitivity of flow cytometry with the precision of PerCP fluorescence, making it an indispensable tool for immunological studies and diagnostics.