Invitrogen™ UltraPure™ Tris Hydrochloride, 500g

Invitrogen™ UltraPure™ Tris Hydrochloride is a high-purity, molecular biology-grade reagent used for buffer preparation in biochemical and molecular biology applications. It is commonly used in nucleic acid gel electrophoresis, blotting, and other buffer-based experiments.

Tris Hydrochloride (tris(hydroxymethyl)aminomethane hydrochloride) provides excellent pH stability, making it ideal for preparing Tris-based buffers such as TAE (Tris-Acetate-EDTA), TBE (Tris-Borate-EDTA), and TE buffer (Tris-EDTA).

Key Features & Benefits

1. High-Purity UltraPure™ Tris Hydrochloride

• Molecular biology-grade purity ensures low contamination levels.
• Highly stable buffering capacity, suitable for long-term experiments.
• Ensures consistent pH control in biological reactions.

2. Versatile Buffering Agent for Molecular Biology

• Used in DNA and RNA electrophoresis buffers (TAE, TBE).
• Suitable for Western blotting and nucleic acid hybridization buffers.
• Helps maintain enzyme activity in molecular and biochemical assays.

3. Convenient & Reliable for Research Applications

• UltraPure™ quality guarantees batch-to-batch consistency.
• Easily dissolves in water, simplifying buffer preparation.
• Long shelf life when stored properly at room temperature.

Specifications

Applications

1. Nucleic Acid Electrophoresis & Blotting

• Key component in TAE and TBE buffers for DNA/RNA electrophoresis.
• Maintains pH stability during gel runs.
• Used in blotting techniques such as Southern and Northern blotting.

2. Biochemical & Molecular Biology Buffers

• Used in TE buffer (Tris-EDTA) for DNA/RNA storage.
• Maintains pH stability in enzyme assays and biochemical reactions.
• Helps preserve protein and nucleic acid integrity in lab workflows.

3. Enzyme Reactions & Protein Research

• Provides optimal buffering for restriction enzyme digestion.
• Stabilizes protein activity in molecular assays.
• Used in chromatography and protein purification techniques.

Pack Size & Catalog Number

Storage & Handling

• Store at room temperature in a dry, well-sealed container.
• Dissolve in deionized water for buffer preparation.
• Adjust pH with HCl or NaOH as needed.

Summary

Invitrogen™ UltraPure™ Tris Hydrochloride is a high-quality buffer reagent used for molecular biology, nucleic acid electrophoresis, and biochemical applications. Its stable pH control and molecular biology-grade purity make it ideal for preparing Tris-based buffers such as TAE, TBE, and TE buffer.