Gibco™ Sodium Pyruvate (100 mM)

The Gibco™ Sodium Pyruvate (100 mM) is a high-quality, sterile, and ready-to-use solution commonly used in cell culture media to supplement carbon sources. This liquid formulation is manufactured under cGMP standards, ensuring consistency and reliability for research and in vitro diagnostic use.

Key Features:

1. Ready-to-Use Format:
   • 100 mM sodium pyruvate solution, formulated with 11 g/L of water, eliminates the need for preparation, saving time and reducing variability.
2. Versatile Supplement:
   • Provides an additional carbon source to support cellular energy metabolism, especially in glycolysis-dependent cells.
3. Sterile and Stable:
   • Sterile-filtered to prevent contamination.
   • Shelf life of 12 months when stored at 2°C to 8°C and protected from light.
4. Manufactured Under cGMP Standards:
   • Produced at two ISO 13485-certified facilities in Grand Island, NY, and Scotland, UK, ensuring supply chain continuity and compliance with regulatory requirements.
5. Flexible Application:
   • While not required for all cell types, sodium pyruvate is recommended for cells previously cultured with this supplement to maintain consistent growth and performance.

Applications:

1. Cell Culture Supplementation:
   • Used as an energy source in media formulations for mammalian cells, particularly for energy-demanding cell lines.
2. Metabolic Studies:
   • Investigates metabolic pathways, including glycolysis, gluconeogenesis, and the tricarboxylic acid (TCA) cycle.
3. Protein Production:
   • Enhances growth and viability of production cell lines, such as CHO and HEK293 cells, during recombinant protein and antibody production.
4. Cell Maintenance:
   • Supports long-term culture and maintenance of mammalian cells requiring supplemental pyruvate for optimal proliferation.

Specifications:

Use Cases:

1. Energy Source in Cell Culture:
   • Enhances cell survival and performance in low-glucose or glucose-depleted media by providing a readily available carbon source.
2. Support for Glycolysis-Dependent Cells:
   • Used in cultures of energy-demanding cells, such as neurons, hepatocytes, or cancer cells, that rely on glycolysis.
3. Metabolic Flexibility in Research:
   • Facilitates experiments exploring cellular metabolic pathways or studying stress responses under altered energy conditions.
4. Custom Media Formulations:
   • Incorporated into specialized media for specific cell culture applications requiring additional metabolic support.

Advantages:

1. Consistency:
   • Manufactured under strict quality controls to ensure uniformity across batches.
2. Convenience:
   • Pre-formulated, sterile, and ready-to-use solution eliminates the need for on-site preparation.
3. Flexibility:
   • Compatible with a wide variety of mammalian cell lines and media formulations.
4. Regulatory Compliance:
   • Produced in ISO 13485-certified and cGMP-compliant facilities for in vitro diagnostic applications.

Storage and Handling:

• Store at 2°C to 8°C, away from direct light to maintain stability and sterility.
• Avoid repeated freeze-thaw cycles to preserve product quality.

Product Details:

The Gibco™ Sodium Pyruvate (100 mM) is an essential supplement for enhancing cell culture performance and supporting energy metabolism. Its high quality, regulatory compliance, and ease of use make it an indispensable tool for cell culture, research, and biomanufacturing applications.