Invitrogen™ Dynabeads™ Untouched™ Human T Cells Kit for 100 preps

The Dynabeads™ Untouched™ Human T-Cells Isolation Kit provides a highly efficient method for isolating pure and viable T-cells from peripheral blood mononuclear cells (PBMC) via negative isolation. This kit is designed to yield untouched T-cells with excellent purity, recovery, and viability, ideal for downstream functional assays and applications.

Key Features

1. Untouched T-Cells:
   • T-cells are isolated without direct contact with beads or antibodies, maintaining their natural state and functionality.
2. High Purity and Viability:
   • Produces T-cells with >95% viability and high recovery rates, suitable for sensitive applications.
3. Simple and Scalable Workflow:
   • No columns are required, enabling easy scalability for larger sample volumes.
4. Wide Application Compatibility:
   • Isolated T-cells are suitable for flow cytometry, functional assays, and other downstream applications.
5. Fast and Efficient:
   • Negative isolation eliminates non-T-cells quickly using Dynabeads™ and magnetic separation.

Specifications

Methodology

1. Preparation:
   • Add the antibody mix (containing monoclonal antibodies against non-T-cells) to the PBMC sample.
2. Binding:
   • The antibodies bind specifically to non-T-cells (e.g., B cells, NK cells, monocytes, dendritic cells, granulocytes, erythrocytes).
3. Bead Addition:
   • Add Depletion Dynabeads™, which bind to the labeled non-T-cells.
4. Magnetic Separation:
   • Use a magnet to separate bead-bound non-T-cells from the untouched T-cells.
5. Isolation:
   • Discard the bead-bound non-T-cells. The remaining untouched T-cells are ready for use.

Contents

• Depletion Dynabeads™: 10 mL
• Antibody Mix: 2 mL, including monoclonal antibodies targeting:
  • CD14, CD16a/b, CD19, CD36, CD56, CD123, CD235a (Glycophorin A)

Applications

1. Flow Cytometry Analysis:
   • Directly analyze isolated T-cells for phenotypic and functional studies.
2. Functional Assays:
   • Perform functional assays such as proliferation, activation, or cytokine secretion studies.
3. Immunological Research:
   • Investigate T-cell-mediated immunity and response.
4. Gene Expression Studies:
   • Extract high-quality RNA or DNA from untouched T-cells for transcriptional or genomic analysis.

Advantages

Comparison with Competitor Kits