Invitrogen™ eBioscience™ Brefeldin A Solution (1000X)

The Invitrogen™ eBioscience™ Brefeldin A Solution (1000X) is a potent reagent designed to inhibit intracellular protein transport. It effectively blocks protein transport to the Golgi complex (GC), causing an accumulation of proteins in the endoplasmic reticulum (ER). This accumulation enhances the detection of intracellular cytokines when cells are activated in vitro and is particularly valuable in intracellular staining protocols followed by flow cytometric analysis.

Key Features:

1. Protein Transport Inhibition:
   • Brefeldin A disrupts protein transport to the Golgi complex, leading to the retention of proteins in the endoplasmic reticulum for enhanced detection of intracellular cytokines.
2. Enhanced Intracellular Cytokine Detection:
   • When used during the final hours of in vitro cell activation, it enables more effective detection of intracellular cytokines in mouse and human cells.
3. Versatility:
   • Effective across a majority of mouse and human cytokines; however, performance may vary by assay system. Its efficacy can be compared with other protein transport inhibitors, such as Monensin.
4. Ease of Use:
   • Supplied in a 1000X concentrated liquid form, it is simple to dilute and incorporate into existing staining workflows.

Specifications:

Applications:

1. Intracellular Staining:
   • Enhances the detection of intracellular cytokines, chemokines, and other proteins in both human and mouse cells.
2. Flow Cytometry:
   • Ideal for intracellular protein analysis in activated cells using multicolor flow cytometry panels.
3. Immunological Research:
   • Used in studies related to immune activation, cytokine profiling, and signaling pathway analysis.
4. Protein Transport Studies:
   • A valuable tool for studying intracellular protein trafficking and Golgi complex function.

Key Benefits:

1. Enhanced Sensitivity:
   • Improves intracellular cytokine detection by preventing secretion during activation.
2. Streamlined Protocol:
   • Easy to integrate into existing intracellular staining workflows, saving time and effort.
3. Compatibility:
   • Suitable for use with both mouse and human cells, providing versatility for various research needs.
4. Reliable Results:
   • Retains intracellular proteins in the ER, ensuring accurate and reproducible results.

Usage Guidelines:

1. Add Brefeldin A to cell cultures during the last hours of in vitro activation.
2. Optimize the concentration and incubation time for specific assay systems.
3. Pair with appropriate permeabilization and fixation buffers for intracellular staining.

Storage and Handling:

• Store at 2–8°C to maintain stability.
• Avoid freezing to prevent loss of activity.

For Research Use Only:

This product is intended for research purposes only and is not suitable for diagnostic or therapeutic applications.