Applied Biosystems™ TaqMan™ Universal PCR Master Mix

The Applied Biosystems™ TaqMan™ Universal PCR Master Mix is a high-performance reagent solution optimized for 5' nuclease DNA applications, including gene expression analysis, genotyping, and miRNA analysis. It is compatible with multiple real-time PCR platforms, ensuring sensitive, accurate, and reliable performance.

Key Features and Benefits

1. Versatile Applications:
   • Designed for gene expression, genotyping, and miRNA analysis.
   • Works effectively with genomic DNA, cDNA, and other DNA samples.
2. AmpliTaq Gold™ DNA Polymerase:
   • Enhanced yield and robust performance for 5' nuclease assays.
   • Provides precise amplification even with G/C-rich sequences.
3. Built-in Passive Reference:
   • Contains ROX dye, ensuring superb precision and normalization on Applied Biosystems real-time PCR systems.
4. Carry-over Contamination Prevention:
   • Incorporates AmpErase™ Uracil-DNA Glycosylase (UNG) to protect against PCR product contamination during subsequent amplifications.
5. Optimized Buffer Components:
   • Includes buffer enhancements to guarantee reliable results across a variety of applications and sample types.
6. Convenient Usage:
   • Ready-to-use 2X concentration minimizes preparation time.
   • Supplied with guidelines to facilitate rapid assay development.
7. Wide Instrument Compatibility:
   • Compatible with a broad range of Applied Biosystems systems, including:
     • 7000, 7300, 7500, 7700, 7900HT Systems
     • GeneAmp 9600
     • Applied Biosystems StepOne™, StepOnePlus™ Systems (Standard Mode).
8. Alternative High-Performance Mix:
   • For faster and advanced capabilities, the TaqMan Fast Advanced Master Mix (Cat. No. 4444963) is available.

Product Composition

The master mix is supplied at 2X concentration and contains:

• AmpliTaq Gold™ DNA Polymerase
• Uracil-DNA Glycosylase (UNG)
• dNTPs with dUTP
• ROX passive reference dye (pre-mixed)
• Optimized buffer components

Specifications

Storage and Shelf Life

• Storage Conditions:
  • Store at 2°C to 8°C in the dark.
  • Ensure no exposure to light to maintain stability.
• Shelf Life:
  • Guaranteed minimum shelf life of 60 days. (Exact expiry printed on the product label and Certificate of Analysis.)

Catalog Numbers and Quantities

Key Applications and Use Cases

1. Gene Expression Analysis:
   • Ideal for measuring mRNA levels across a variety of research settings.
   • Suitable for studying transcriptional regulation and expression profiles.
2. Genotyping:
   • Enables accurate differentiation between alleles in genetic analysis.
3. miRNA Analysis:
   • Facilitates detection and quantification of microRNA molecules for functional studies.
4. High-Throughput Laboratories:
   • The scalable format and compatibility with automated systems support high-throughput workflows.

Performance Enhancements

1. Reliable Amplification:
   • Provides robust amplification with minimal optimization, even in challenging templates such as G/C-rich sequences.
2. Reduced Contamination:
   • Integrated UNG minimizes false positives caused by carry-over contamination.
3. Precision in Real-Time PCR:
   • Built-in ROX dye ensures accurate normalization and reproducible results.

Additional Notes

• Safety: For Research Use Only. Not for use in diagnostic procedures.
• Alternative Product: For faster reactions and enhanced performance, use TaqMan Fast Advanced Master Mix