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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreen)
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Reading Options: Top and Bottom
No. of dispensers: 2
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBLATGD2) represents the highest configuration in the Varioskan LUX series—offering maximum flexibility, dual reagent dispensing, and integrated gas control for advanced cell-based, kinetic, and multimode assay applications.
Engineered for researchers demanding precision and reproducibility, the Varioskan™ LUX integrates five major detection technologies—Absorbance (UV-Vis), Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence (TRF), and AlphaScreen/AlphaLISA—within one modular platform. The system’s dual dispensers enable simultaneous or sequential reagent addition for real-time reaction monitoring, while the gas module maintains optimal CO₂ (0.1–15%) and O₂ (1–21%) concentrations for physiological assay conditions.
With automatic dynamic range selection, spectral scanning, smart safety controls, and SkanIt™ Software (Research Edition included), the Varioskan LUX simplifies complex workflows while ensuring accurate and traceable results every time.
Key Features
- Five integrated detection technologies: Absorbance, Fluorescence Intensity (including FRET), Luminescence (direct and filtered), AlphaScreen/AlphaLISA, and Time-Resolved Fluorescence (TR-FRET/hTRF).
- Dual onboard dispensers: Independent channels (2 µL–5 000 µL) for fast, accurate liquid handling during kinetic assays.
- Integrated Gas Control Module: Maintains CO₂ and O₂ levels for live-cell and metabolic studies.
- Automatic Dynamic Range Selection: Automatically adjusts gain to prevent signal saturation.
- Filter and Monochromator Optics: Combine flexibility with spectral precision for assay optimization.
- Smart Safety Controls: Built-in diagnostics and error prevention ensure uninterrupted operation.
- Validated Compatibility: Certified HTRF™ reader (Cisbio Bioassays) and DLReady™ validated (Promega Corporation).
- Upgradeable, modular design: Expands with laboratory needs, ensuring long-term investment value.
- Seamless automation integration: Compatible with robotic systems and plate handlers.
SkanIt™ Software (Research Edition Included)
- License-free installation for multiple users.
- Virtual Pipetting Tool for defining plate layouts with ease.
- Real-time visualization of kinetic and spectral data.
- Continuous autosave to prevent data loss from interruptions.
- Comprehensive data processing: blank subtraction, SD/CV%, standard curve, dose-response, kinetic and spectral analysis, custom formulas, and QC evaluation.
- Instant export options: *.xlsx, *.pdf, *.xml, *.txt — or automatic email delivery after run completion.
- Traceable audit trail for all run-time actions.
- Optional Drug Discovery Edition supports FDA 21 CFR Part 11 compliance for regulated environments.
Applications
- High-throughput cell-based assays requiring CO₂/O₂ regulation
- Enzyme kinetics and reaction onset monitoring with dual dispensing
- AlphaScreen and AlphaLISA proximity assays
- FRET and TR-FRET/hTRF interaction studies
- ELISA and nucleic acid quantification
- Reporter-gene, ATP, and luminescence-based assays
- Spectral scanning and assay development optimization
Technical Specifications
| Parameter | Specification |
|---|---|
| Catalog Number | VLBLATGD2 |
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence, AlphaScreen |
| Reading Options | Top and Bottom |
| Dispensers | 2 (Volume range: 2–5 000 µL, 1 µL increments) |
| Gas Control Module | CO₂: 0.1–15 %; O₂: 1–21 % |
| Measurement Modes | Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra |
| Spectral Scanning Speed | < 2.2 s/well (400–500 nm, 2 nm step) |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | FI > 6 decades; Lum > 7 decades; TRF > 6 decades |
| Sensitivity | FI (top) < 0.4 fmol fluorescein/well; FI (bottom) < 4 fmol fluorescein/well; TRF < 1 amol/well; Lum < 7 amol ATP/well; AlphaScreen < 100 amol phosphotyrosine/well |
| Photometric Accuracy | 0.003 Abs or ±2 % (200–399 nm, 0–2 Abs); ±1 % (400–1000 nm, 0–3 Abs) |
| Linearity | ±2 % (96-well 0–4 Abs @ 450 nm; 384-well 0–3 Abs) |
| Precision | SD < 0.001 Abs or CV < 0.5 % (450 nm, 0–3 Abs) |
| Wavelength Range | Abs 200–1000 nm; FI Exc. 200–822 nm / Em. 270–840 nm; TRF Exc. 334 nm / Em. 400–700 nm; Lum 360–670 nm; AlphaScreen Exc. 680 nm / Em. 400–660 nm |
| Plate Compatibility | 6 to 1536 wells (Absorbance: 6–384 wells); μDrop plates |
| Incubator Temperature | Ambient +4 °C to 45 °C |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp and LED |
| Shaking | Orbital |
| Dimensions (L × W × H) | 58 × 53 × 51 cm (23 × 21 × 20 in.) |
| Weight | 57 kg (125 lb) |
| Interface | Thermo Scientific™ SkanIt™ Software |
| Product Line | Varioskan™ |
| Read-out Range | 0–6 Abs |
| Included Accessories | Gas Module, Dual Dispensers, SkanIt PC Software (Research Edition), Nunc™ 96-Well Optical-Bottom Microplate (black, TC surface) |
Unique Advantages
- Dual dispenser configuration enables rapid substrate addition and complex kinetic studies.
- True physiological control with integrated CO₂/O₂ regulation—ideal for live-cell assays.
- Unmatched sensitivity and dynamic range across all five measurement technologies.
- Fully automated workflow with self-diagnostics, gain optimization, and calibration.
- Compatible with robotics and plate handlers for high-throughput screening.
- Future-proof modularity—expand detection modes and accessories as needs evolve.
Ordering Information
| Catalog Number | Measurement Technologies | Reading Options | Dispensers | Includes |
|---|---|---|---|---|
| VLBLATGD2 | Absorbance, Fluorescence Intensity, Luminescence, TRF, AlphaScreen | Top and Bottom | 2 | Gas Module, Dual Dispensers, SkanIt PC Software (Research Edition) |
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBLATGD2) is the flagship model in the Varioskan™ series, combining precision optics, dual reagent dispensing, and environmental control for unparalleled assay versatility. Its design supports everything from routine ELISAs to sophisticated kinetic and cell-based studies under physiological gas conditions. Backed by Thermo Scientific’s innovation and reliability, the Varioskan™ LUX empowers laboratories to achieve consistent, high-quality data across every experimental workflow.