0
Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreen)
Catalog No :
CAS Number :
Brand :
In Stock
Reading Options: Top and Bottom
No. of dispensers: 0
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (Model VLBLATGD0) is a high-performance, modular detection platform designed for life-science researchers and assay developers who require flexible, accurate, and reproducible multimode analysis.
Equipped with five advanced detection technologies—Absorbance (UV-Vis), Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence (TRF), and AlphaScreen/AlphaLISA—the Varioskan™ LUX delivers outstanding sensitivity and reliability for endpoint, kinetic, and spectral assays.
This model includes an integrated Gas Control Module for precise CO₂ (0.1–15 %) and O₂ (1–21 %) regulation, making it ideal for cell-based applications that demand a physiologically controlled microenvironment. Smart safety systems, automated calibration, and powerful SkanIt™ Software combine to make operation intuitive and error-free, even in complex workflows.
Key Features
- Five detection modes in one instrument: Absorbance, Fluorescence Intensity (FRET compatible), Luminescence, AlphaScreen/AlphaLISA, and TR-FRET/hTRF.
- Integrated Gas Module: Precisely controls CO₂ and O₂ concentrations for live-cell and hypoxia assays.
- Automatic Dynamic Range Selection: Optimizes gain for each well—no manual adjustments needed.
- Filter + Monochromator Optics: Provides flexibility for both fixed-wavelength and spectral-scanning assays.
- Spectral Scanning: High-speed (< 2.2 s/well) wavelength scanning for assay optimization.
- Smart Safety Controls: Built-in sensors detect and alert users to potential issues before measurement begins.
- Automated Calibration & Self-Diagnostics: Ensures consistent, long-term performance.
- Validated Compatibility: Certified HTRF™ reader (Cisbio Bioassays) and DLReady™ validated (Promega).
- Modular & Upgradeable: Designed for expansion with optional dispensers or advanced assay modules.
SkanIt™ Software (Research Edition Included)
- License-free installation for unlimited users.
- Virtual Pipetting Tool simplifies plate layout setup.
- Real-time data visualization of kinetic curves and spectra.
- Continuous autosave protects results during unexpected interruptions.
- Built-in data processing: blank subtraction, SD/CV %, standard & dose-response curves, kinetic/spectral analysis, and user-defined formulas.
- Flexible data export: *.xlsx, *.pdf, *.xml, *.txt, or automatic email reporting after run completion.
- Traceable audit reports with full runtime history.
- Optional Drug Discovery Edition supports FDA 21 CFR Part 11 compliance.
Applications
- Cell-based assays requiring controlled CO₂/O₂ conditions
- ELISA, protein, and nucleic-acid quantification
- Enzyme kinetics and signal-onset monitoring
- FRET/TR-FRET (hTRF) and AlphaScreen/AlphaLISA assays
- Reporter-gene and ATP-based luminescence assays
- Spectral scanning and assay development optimization
- Drug screening and cytotoxicity testing
Technical Specifications
| Parameter | Specification |
|---|---|
| Catalog Number | VLBLATGD0 |
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence, AlphaScreen |
| Reading Options | Top and Bottom |
| Dispensers | None |
| Gas Control Module | CO₂ 0.1–15 %; O₂ 1–21 % |
| Wavelength Range | Abs 200–1000 nm; Fluorescence Exc. 200–822 nm / Em. 270–840 nm; TRF Exc. 334 nm / Em. 400–700 nm; Lum 360–670 nm; AlphaScreen Exc. 680 nm / Em. 400–660 nm |
| Spectral Scanning Speed | < 2.2 s/well (400–500 nm, 1 flash, 2 nm step) |
| Measurement Modes | Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | FI > 6 decades (top); Lum > 7 decades; TRF > 6 decades |
| Sensitivity | FI (top) < 0.4 fmol fluorescein/well; FI (bottom) < 4 fmol; TRF < 1 amol/well; Lum < 7 amol ATP/well; AlphaScreen < 100 amol phosphotyrosine/well |
| Photometric Accuracy | 0.003 Abs or ±2 % (200–399 nm, 0–2 Abs); ±1 % (400–1000 nm, 0–3 Abs) |
| Linearity | ±2 % (96-well 0–4 Abs @ 450 nm; 384-well 0–3 Abs) |
| Precision | SD < 0.001 Abs or CV < 0.5 % (450 nm, 0–3 Abs) |
| Plate Compatibility | 6–1536 wells (Abs 6–384 wells); μDrop plates |
| Incubator Temperature | Ambient + 4 °C to 45 °C |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp and LED |
| Shaking | Orbital |
| Dimensions (L × W × H) | 58 × 53 × 51 cm (23 × 21 × 20 in.) |
| Weight | 57 kg (125 lb) |
| Interface | Thermo Scientific™ SkanIt™ Software |
| Product Line | Varioskan™ |
| Read-out Range | 0–6 Abs |
| Included Accessories | Gas Module, SkanIt PC Software (Research Edition), Nunc™ 96-Well Optical-Bottom Microplate (black, TC surface) |
Unique Advantages
- Physiological assay conditions: Built-in gas control ensures optimal CO₂/O₂ for live-cell assays.
- Unparalleled detection sensitivity: Measures from amol to µmol concentrations with linear accuracy.
- Automated performance checks: Built-in calibration and diagnostics maintain consistency over years of use.
- Flexible assay compatibility: Supports a wide range of microplate formats from 6 to 1536 wells.
- Trusted reliability: Validated by industry leaders (Cisbio HTRF™, Promega DLReady™).
Ordering Information
| Catalog Number | Measurement Technologies | Reading Options | Dispensers | Includes |
|---|---|---|---|---|
| VLBLATGD0 | Absorbance, Fluorescence Intensity, Luminescence, TRF, AlphaScreen | Top and Bottom | None | Gas Module and SkanIt PC Software (Research Edition) |
The Varioskan™ LUX Multimode Microplate Reader (VLBLATGD0) combines precision optics, controlled atmospheric conditions, and intelligent automation for unmatched assay versatility. Its integrated gas module supports physiological environments, making it the ideal choice for advanced cell-based, kinetic, and spectral applications. Reliable, modular, and future-ready—Varioskan™ LUX sets a new standard for scientific measurement excellence.