Vircell parainfluenza IgG/IgM Elisa kit

The Vircell Parainfluenza IgG/IgM ELISA Kit is a high-performance indirect immunoenzyme assay designed for the detection of IgG and IgM antibodies against Parainfluenza virus in human serum or plasma. This versatile kit enables testing for Parainfluenza virus types 1, 2, and 3, providing accurate results for clinical diagnostics and research.

Key Features

1. Dual IgG/IgM Detection:
   • Enables simultaneous determination of IgG and IgM antibodies, supporting comprehensive serological profiling.
2. Simplified Sample Handling:
   • Serum dilution and sorbent treatment (when necessary) are performed directly in the well, eliminating the need for pre-dilution.
3. Automation Compatibility:
   • Fully compatible with automated ELISA systems for high-throughput testing.
4. Ready-to-Use Reagents:
   • Coloured, liquid reagents reduce handling errors and improve workflow efficiency.
5. Flexible Plate Design:
   • Break-apart, colour-coded wells enable customized testing based on sample size and volume.
6. Stable Performance:
   • Lyophilized conjugates (when required) ensure long-term reagent stability and consistent assay performance.

Available Product Variants

Principle of the Assay

The Vircell Parainfluenza ELISA Kit employs an indirect ELISA methodology:

1. Antigen Binding:
   • Parainfluenza antigens are adsorbed onto the polystyrene wells of the ELISA plate.
2. Sample Interaction:
   • Antibodies in the sample bind to the adsorbed antigens. Unbound immunoglobulins are washed off.
3. Conjugate Reaction:
   • An enzyme-labeled anti-human globulin binds to the antigen-antibody complex.
4. Signal Development:
   • TMB substrate is added, producing a blue-colored solution. Upon stopping the reaction, the color changes to yellow.
5. Quantification:
   • The optical density (OD) is measured at 450 nm, and antibody levels are determined based on OD values.

Applications and Use Cases

1. Clinical Diagnostics:

• Acute Infection Detection:
  • Detect IgM antibodies to confirm recent Parainfluenza virus infections.
• Immunity Assessment:
  • Measure IgG antibodies to evaluate immunity from past infections or vaccination.

2. Epidemiological Studies:

• Seroprevalence Monitoring:
  • Assess Parainfluenza virus exposure in populations.
• Outbreak Investigations:
  • Identify infection patterns during outbreaks of respiratory diseases.

3. Vaccine Research:

• Post-Vaccination Studies:
  • Evaluate the immune response and efficacy of Parainfluenza vaccines.
• Efficacy Testing:
  • Measure antibody production after vaccination.

4. Laboratory Research:

• Immunological Studies:
  • Explore immune responses to Parainfluenza virus infections.
• Antibody Profiling:
  • Differentiate IgG and IgM responses to Parainfluenza types 1, 2, and 3.

Kit Components

Assay Specifications

Advantages

1. Dual Detection Capability:
   • Simultaneously detect IgG and IgM antibodies for comprehensive serological profiling.
2. Simplified Handling:
   • No pre-dilution required; reagents are ready-to-use for faster workflows.
3. Flexible Testing:
   • Break-apart wells allow for customizable sample testing and batch sizes.
4. High Sensitivity and Specificity:
   • Reliable detection of antibodies against Parainfluenza virus types 1, 2, and 3.
5. Automation-Ready:
   • Fully compatible with automated systems for high-throughput diagnostics.

Storage and Stability

• Storage Conditions: Store all components at 2–8°C.
• Shelf Life: Use before the expiration date indicated on the product label.

The Vircell Parainfluenza IgG/IgM ELISA Kit is a versatile and reliable tool for detecting antibodies against Parainfluenza virus types 1, 2, and 3 in human serum or plasma. Its flexibility, automation compatibility, and dual detection capabilities make it an excellent choice for clinical diagnostics, epidemiological studies, and vaccine research.