Vircell Influenza A Elisa IgG/IgM Elisa Kit

The Vircell Influenza ELISA IgG/IgM Kit is a high-performance indirect immunoenzyme assay designed for the detection and differentiation of IgG and IgM antibodies against influenza virus in human serum or plasma. This kit offers flexibility, efficiency, and automation compatibility, making it an essential tool for laboratories involved in influenza diagnostics and research.

Key Features

1. Dual IgG/IgM Determination:
   • Allows for the detection of IgG and IgM antibodies in the same assay, providing comprehensive serological profiling.
2. Simplified Sample Handling:
   • No pre-dilution required; serum dilution and sorbent treatment (if necessary) are performed directly in the well.
3. Automation Compatibility:
   • Suitable for automated ELISA systems, enabling high-throughput testing.
4. User-Friendly Design:
   • Pre-coated plates, break-apart wells, and ready-to-use reagents simplify workflows and minimize handling errors.
5. Reliable Performance:
   • Provides sensitive and specific detection of antibodies against Influenza A and Influenza B.

Available Product Variants

Principle of the Assay

The Vircell Influenza ELISA IgG/IgM Kit employs an indirect ELISA method for detecting influenza-specific antibodies:

1. Antigen Binding:
   • Influenza antigens are adsorbed onto the surface of the ELISA plate wells.
2. Antibody Binding:
   • Antibodies in the sample bind to the adsorbed influenza antigens.
   • Unbound immunoglobulins are washed off.
3. Conjugate Reaction:
   • An enzyme-labeled anti-human globulin binds to the antigen-antibody complex.
4. Signal Development:
   • TMB substrate is added, producing a blue-colored solution. Upon stopping the reaction, the color changes to yellow.
5. Quantification:
   • The optical density (OD) is measured at 450 nm, and the results indicate the presence of IgG or IgM antibodies against influenza.

Applications and Use Cases

1. Clinical Diagnostics:

• Acute Infection Detection:
  • Measure IgM antibodies to identify recent influenza infections.
• Immunity Assessment:
  • Quantify IgG antibodies to evaluate immunity from past infections or vaccination.

2. Vaccine Research:

• Serological Monitoring:
  • Evaluate immune responses to influenza vaccines.
• Efficacy Studies:
  • Assess antibody production post-vaccination.

3. Epidemiological Studies:

• Influenza Surveillance:
  • Monitor seroprevalence and antibody trends in populations.
• Outbreak Investigations:
  • Identify infection patterns and immunity gaps during outbreaks.

4. Laboratory Research:

• Viral Immunology:
  • Study immune responses to influenza virus infections.
• Antibody Profiling:
  • Differentiate IgG and IgM responses to Influenza A and B.

Kit Components

Assay Specifications

Advantages

1. Dual Detection Capability:
   • Simultaneously detect IgG and IgM antibodies for comprehensive serological profiling.
2. Efficient Workflow:
   • No pre-dilution required; reagents are ready-to-use for faster processing.
3. Flexible Testing:
   • Break-apart wells accommodate different sample sizes and batch testing needs.
4. High Sensitivity and Specificity:
   • Reliable detection of antibodies against both Influenza A and Influenza B.
5. Automation-Ready:
   • Compatible with automated systems for high-throughput testing.

Storage and Stability

• Storage Conditions: Store all components at 2–8°C.
• Shelf Life: Use before the expiration date indicated on the product label.

The Vircell Influenza ELISA IgG/IgM Kit is a versatile and reliable tool for detecting and quantifying influenza-specific antibodies. Its dual detection capability, automation compatibility, and user-friendly design make it an excellent choice for clinical diagnostics, vaccine research, and epidemiological studies.