Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific™ Maxima H Minus Reverse Transcriptase (RT) is a next-generation, thermostable reverse transcriptase engineered through in vitro evolution of Moloney Murine Leukemia Virus (M-MuLV) RT. The enzyme maintains both RNA-dependent and DNA-dependent polymerase activities while lacking RNase H activity due to a targeted mutation in the RNase H domain. This unique design enables efficient synthesis of high-quality, full-length cDNA up to 20 kb, even from complex or highly structured RNA templates.

With up to 50-fold higher processivity than wild-type M-MuLV RT, Maxima H Minus RT delivers faster synthesis rates, exceptional robustness, and consistent performance across a wide range of RNA inputs. Its enhanced thermostability allows reactions to be performed at temperatures up to 65 °C, improving specificity and reducing the impact of RNA secondary structures.

Key Features

• High thermostability – retains ~90% activity after 60 minutes at 50 °C
• Active up to 65 °C – ideal for GC-rich or highly structured RNA templates
• RNase H–minus formulation – enables synthesis of long, intact cDNA up to 20 kb
• Ultra-high processivity – approximately 50× higher than wild-type M-MuLV RT
• Rapid reaction kinetics – complete cDNA synthesis in 15–30 minutes
• Exceptional sensitivity – reproducible results from 1 pg to 5 µg total RNA
• Enhanced inhibitor tolerance – resistant to guanidine, formamide, and ethanol
• Modified nucleotide compatibility – suitable for labeled cDNA applications

Applications

• First-strand cDNA synthesis for RT-PCR and RT-qPCR
• Reverse transcription at elevated temperatures to overcome RNA secondary structures
• cDNA synthesis for cloning and gene expression analysis
• Preparation of labeled cDNA probes for microarray applications
• RNA analysis using primer extension methods

Product Formats & Ordering Information

Technical Specifications

Contents & Storage

Each package contains:

• Maxima H Minus Reverse Transcriptase – 200 U/µL
• 5× RT Buffer

Store at –20 °C. Avoid repeated freeze–thaw cycles.

Thermo Scientific™ Maxima H Minus Reverse Transcriptase is an ideal solution for high-fidelity, long-length, and rapid cDNA synthesis in demanding molecular biology workflows. Its exceptional thermostability, ultra-high processivity, and resistance to common reaction inhibitors make it particularly well suited for RT-PCR, RT-qPCR, cloning, and transcriptome analysis, even when working with low-abundance or structurally complex RNA. For researchers seeking consistent, high-yield, and full-length cDNA synthesis, Maxima H Minus RT delivers reliable performance and superior results.