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Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Filter Plates
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Sample Volume: 40 to 100 μL
Specifications:
| Application | Size Exclusion Chromatography |
| Storage Temperature | 2-8°C |
| Product Type | columns |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Filter Plates offer a fast, reliable, and high-throughput method for removing non-conjugated dyes, biotinylation reagents, crosslinkers, and reducing agents from protein labeling reactions. Each 96-well plate is pre-packed with a proprietary 7K MWCO size-exclusion resin, allowing for small-molecule cleanup from protein samples >7 kDa while maintaining exceptional protein recovery rates. This product is ideal for labs processing multiple samples in parallel, such as in antibody labeling workflows, proteomics studies, and assay development.
Key Features and Benefits
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High-Efficiency Clean-Up
Efficiently removes non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers within 15 minutes. -
Exceptional Protein Recovery
Low-binding resin ensures maximum retention of labeled or native protein samples, even at low concentrations (as low as 25 μg/mL). -
High-Throughput Format
The 96-well filter plate format enables simultaneous processing of multiple samples, improving throughput and reproducibility. -
Ready to Use
Pre-dispensed resin eliminates the need for column packing or equilibration—compatible with standard benchtop centrifuges. -
Consistent Results
Achieves ≥95% small molecule removal with low well-to-well variability—ideal for standardized workflows.
Applications
- Post-Labeling Clean-Up of Proteins and Antibodies
- Removal of Non-Conjugated Fluorescent Dyes and Biotin
- Purification Following Reductive Crosslinking
- Sample Preparation for Accurate Dye-to-Protein Ratio Quantification
- Pre-analysis Cleanup for Proteomics or Spectroscopic Assays
Available Formats
| Catalog Number | Quantity | Format | Sample Volume (per well) |
|---|---|---|---|
| A44304 | 2 Plates | 96-well Filter Plate | 40 to 100 μL |
| A44305 | 2 × 2 Plates | 96-well Filter Plate | 40 to 100 μL |
Specifications
| Property | Detail |
|---|---|
| Column Type | Proprietary size-exclusion resin (7K MWCO) |
| Format | 96-well spin filter plate |
| Sample Compatibility | Proteins >7 kDa |
| Sample Volume per Well | 40 to 100 μL |
| Processing Time | <15 minutes |
| Storage Conditions | 4°C (Do not freeze) |
| Shipping Condition | Ambient |
| Product Line | Zeba™ |
| Unit Size | Each (2 or 4 plates depending on pack size) |
| Application | Small-molecule cleanup post protein labeling |
The Zeba™ Dye and Biotin Removal Spin Filter Plates offer a streamlined, high-throughput solution for post-labeling protein cleanup. Whether you're removing free dyes, biotin, crosslinkers, or reducing agents, these plates ensure fast and reproducible purification with minimal sample loss. Designed for labs handling large sample batches, they are a versatile, easy-to-use tool for achieving high-purity results in protein research, antibody development, and diagnostic assay preparation.
- Pack Size: 2/pk 4/pk