Thermo Scientific™ Pierce™ Protein A/G Agarose

Thermo Scientific™ Pierce™ Protein A/G Agarose is a versatile, high-performance affinity resin designed for the efficient purification of IgG antibodies from a wide range of mammalian species. It features a recombinant fusion protein that combines the immunoglobulin-binding domains of Protein A and Protein G, covalently immobilized onto high-quality, crosslinked 6% beaded agarose (CL-6B). This configuration allows the resin to exhibit broad binding specificity and high capacity, making it ideal for applications requiring robust and reproducible IgG purification.

Key Features & Benefits

• Broad IgG Binding Spectrum: Binds all subclasses of mammalian IgG, including mouse IgG1, IgG2a, IgG2b, IgG3, and human IgG1–IgG4.
• Fusion Ligand Format: Contains four Fc-binding domains from Protein A and two from Protein G for enhanced subclass binding efficiency.
• High Purification Yield: Binding capacity of >7 mg human IgG per mL of resin.
• Stable and Inert Matrix: Uses crosslinked 6% CL-6B agarose with leach-resistant, covalent immobilization via Thermo Scientific AminoLink™ chemistry.
• Reusable Resin: Can be regenerated and reused multiple times with minimal loss in performance.
• Reduced Nonspecific Binding: Engineered to avoid interaction with serum albumin and other non-target proteins.

Applications

• Purification of mouse monoclonal antibodies from culture supernatants or ascites fluid.
• Isolation of polyclonal IgG antibodies from complex mammalian sera.
• Capture of IgG from multiple species when subclass or isotype is unknown.
• Use in immunoprecipitation or immunoaffinity chromatography.

Technical Specifications

Advantages of Protein A/G Agarose Over Individual A or G Resins

• Combines the strong subclass-specific affinities of both Protein A and Protein G.
• Eliminates the need to pre-characterize antibody isotype or subclass.
• More robust binding of IgG from species with variable affinity to Protein A or G alone.

Pierce™ Protein A/G Agarose delivers broad-based IgG purification with high binding efficiency and stability. Its fusion ligand format and robust agarose matrix make it the optimal choice for researchers working with unknown or mixed antibody subclasses across various mammalian species. Whether in small-scale lab work or preparative immunoaffinity workflows, this resin ensures consistent, high-yield performance for antibody purification and analysis.