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Thermo Scientific™ Pierce™ Protein A Chromatography Cartridges
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Binding Capacity: ≥ 35mg human IgG/ml of resin bed
Specifications:
| Application | Protein Assays & Analysis, chromatography |
| Storage Temperature | 2-8°C |
| Product Type | Molecular Biology Reagent |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
Thermo Scientific™ Pierce™ Protein A Chromatography Cartridges are ready-to-use, pre-packed affinity columns designed for efficient purification of IgG antibodies from serum, ascites fluid, and cell culture supernatants. These cartridges are pre-filled with Protein A Plus Agarose, a high-capacity resin that combines native Protein A with crosslinked 6% beaded agarose (CL-6B) for high-yield and high-purity antibody recovery.
Engineered for both manual and automated workflows, these cartridges are compatible with syringes, peristaltic pumps, and major FPLC/ÄKTA™ systems. Each cartridge includes a connection accessory pack for Luer-Lok or 1/16” tubing fittings.
Key Features & Benefits
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Pre-Packed, Ready-to-Use Cartridges
Simplify your antibody purification workflow with high-reproducibility and reduced setup time. -
High Binding Capacity
Binds >34 mg human IgG/mL resin; ideal for purifying large antibody quantities. -
Stable & Reusable Resin
Low leaching, covalent ligand attachment ensures stability through multiple purification cycles. -
Versatile Species Compatibility
Binds strongly to human, rabbit, pig, dog, and cat IgG subclasses. Moderate to weak binding to some mouse IgG subclasses (not recommended for IgG1). -
AminoLink™ Coupling Chemistry
Provides stable amide bond linkage between Protein A and agarose beads; minimizes nonspecific binding. -
Flexible Integration
Directly connects to chromatography systems or syringes without additional hardware.
Resin & Cartridge Specifications
| Feature | Details |
|---|---|
| Stationary Phase | Protein A Plus Agarose |
| Resin Base | Crosslinked 6% beaded agarose (CL-6B) |
| Protein A Source | Native (Staphylococcus aureus-derived) |
| Ligand Immobilization Method | AminoLink Coupling Chemistry (amide bonds) |
| Binding Capacity | >34 mg/mL (Human IgG) |
| Column Format | Cartridges: 1 mL or 5 mL |
| Compatibility | Syringe, FPLC, ÄKTA™, peristaltic pump |
| Reusability | Yes – regenerable |
| Storage Conditions | 4°C to 8°C, do not freeze |
Applications
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Antibody Purification
- Polyclonal and monoclonal IgG from serum, ascites, and hybridoma culture media
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Protein A Affinity Capture
- Efficient recovery of Fc-region binding IgG subclasses
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Small- to Mid-Scale Chromatography
- Suitable for laboratory-scale and pilot-scale purifications
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Immunology & Proteomics Workflows
- Downstream use in ELISA, Western blot, conjugation, and labeling
Thermo Scientific™ Pierce™ Protein A Chromatography Cartridges deliver high-efficiency IgG purification with a format that suits both automated systems and manual benchwork. Backed by high-binding-capacity resin, low nonspecific binding, and stable ligand attachment, these cartridges are a trusted solution for research labs involved in antibody production, validation, and downstream analysis.
- Pack Size: 2 cartridges x 1 mL 1 cartridge x 5 mL