Thermo Scientific™ Phorbol-12-myristate-13-acetate 97%

Thermo Scientific™ Phorbol-12-myristate-13-acetate (PMA), also known as TPA, is a highly pure phorbol ester with ≥96.0% purity by HPLC. It is a potent activator of protein kinase C (PKC) and is widely used in molecular biology, cell signaling, cancer research, and immunology studies. With its ability to mimic diacylglycerol (DAG), PMA serves as a key reagent for investigating signal transduction pathways, cell differentiation, and immune responses.

Key Features:

1. High Purity:
   • HPLC-verified ≥96.0% purity, ensuring consistent experimental results.
2. PKC Activation:
   • Potent activator of protein kinase C (PKC), making it essential for studying signal transduction pathways.
3. Versatile Research Applications:
   • Used for cell differentiation, immune response stimulation, and tumor-promoting activity studies.
4. Stable Formulation:
   • Supplied as a colorless to light yellow film or foam, ensuring long-term stability when stored at recommended conditions.
5. Convenient Packaging:
   • Available in 1 mg and 5 mg glass bottles for flexible usage in various experimental scales.

Specifications:

Applications:

1. Signal Transduction Studies:
   • Activates PKC to explore intracellular signaling pathways and downstream cellular effects.
2. Immune Cell Stimulation:
   • Stimulates immune cells such as T-cells, macrophages, and neutrophils to investigate cytokine production and immune regulation.
3. Cancer Research:
   • Serves as a tumor promoter to study oncogenesis, tumor progression, and therapeutic drug efficacy.
4. Oxidative Stress Research:
   • Induces reactive oxygen species (ROS) production for studying oxidative stress and antioxidant enzyme responses.
5. Cell Differentiation and Proliferation:
   • Promotes differentiation in cell lines such as THP-1 monocytes into macrophages and stimulates T-cell activation.
6. Protein Kinase C Studies:
   • Models diacylglycerol (DAG)-mediated PKC activation, enabling insights into enzyme function and receptor signaling.

Protocol Overview:

1. Reconstitution:
   • Dissolve PMA in DMSO or another water-miscible organic solvent to prepare a stock solution.
2. Dilution:
   • Dilute stock solution into the desired buffer or cell culture medium for experimental use.
3. Application:
   • Add prepared solution to cells or reaction mixtures according to experimental protocols.
4. Storage:
   • Store unused PMA or stock solution at −20°C and protect from light to maintain stability.

Ordering Information:

Storage and Handling:

• Storage:
  • Keep at −20°C or below.
• Stability:
  • Protect from light to prevent degradation.
• Solubility:
  • Soluble in DMSO and other water-miscible organic solvents.

Safety Information:

• Hazards:
  • PMA exhibits tumor-promoting activity and may induce inflammatory responses.
• Precautions:
  • Handle with care using appropriate PPE (gloves, lab coat, goggles).
  • Work in a well-ventilated area or fume hood.

Related Products:

1. Ionomycin Calcium Salt:
   • Used with PMA for T-cell activation.
2. ROS Detection Assays:
   • Quantify reactive oxygen species production.
3. PKC Inhibitors:
   • Study PKC inhibition alongside PMA activation.

Regulatory Notes:

• For Research Use Only: Not intended for diagnostic or therapeutic applications.

The Thermo Scientific™ Phorbol-12-myristate-13-acetate (PMA) offers exceptional quality and versatility for studying cellular signaling, immune response, and cancer biology. Its high purity and consistent performance make it a preferred choice for advanced molecular biology and biomedical research applications.