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Thermo Scientific™ Phenol:Chloroform + Tris Buffer
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
The Thermo Scientific™ Pierce Phenol-Chloroform + Tris Buffer is a high-quality, ready-to-use solution designed for efficient nucleic acid purification. This reagent combines phenol premixed with chloroform
alcohol (25:24:1, v/v) and includes a Tris buffer for pH adjustment, making it a versatile tool for DNA extraction applications.
Key Features
- Ready-to-Use Solution:
- Premixed phenol and chloroformalcohol (25:24:1) for immediate application in nucleic acid extractions.
- pH Adjustment Flexibility:
- Supplied at pH 6.7 for specialized DNA extraction workflows; includes Tris buffer to adjust to pH 8.0 for standard DNA applications.
- Nuclease-Free Performance:
- Certified free of DNases and RNases for contamination-free extractions.
- Optimized Composition:
- Chloroform denatures proteins, while isoamyl alcohol reduces foam formation during mixing.
- High Versatility:
- Compatible with various sample types, including cells, tissues, and biological fluids.
Specifications
| Feature | Details |
|---|---|
| Product Type | Phenol-Chloroform and Tris Buffer |
| Form | Liquid |
| pH | 6.7 (adjustable to 8.0 with supplied buffer) |
| Quantity | 400 mL |
| Applications | DNA Extraction |
| Unit Size | 100 mL or 400 mL |
| Storage Conditions | 2°C to 8°C |
| Shipping Condition | Ambient |
Applications
- DNA Extraction:
- Isolate high-quality DNA by separating proteins and contaminants during phenol-chloroform extraction.
- RNA Preparation:
- Use for RNA extractions with acidic pH adjustments (e.g., pH 6.7).
- Sample Purification:
- Ideal for removing proteins and other contaminants from nucleic acid samples.
Advantages
| Feature | Benefit |
|---|---|
| Premixed Convenience | Reduces preparation time and minimizes handling errors. |
| Adjustable pH | Flexibility for both DNA and RNA applications. |
| Nuclease-Free Certification | Ensures contamination-free nucleic acid purification. |
| Comprehensive Packaging | Includes buffer for precise pH adjustment. |
| Optimized Stability | Long shelf-life when stored at recommended conditions. |
Comparison with Competitor Products
| Feature | Thermo Scientific Pierce Phenol-Chloroform | Invitrogen UltraPure Phenol-Chloroform | Sigma-Aldrich Phenol-Chloroform |
|---|---|---|---|
| Premixed Formulation | Yes | Yes | Yes |
| Adjustable pH | Yes (6.7 to 8.0) | Fixed at pH 8.05 | Fixed at pH 8.0 |
| Nuclease-Free Certification | Yes | Yes | Yes |
| Included Tris Buffer | Yes | No | No |
| Storage Conditions | 2°C to 8°C | 2°C to 8°C | 2°C to 8°C |
| Packaging | 100 mL & 400 mL bottle | 100 mL bottle | 400 mL bottle |
| Applications | DNA/RNA extraction | DNA/RNA extraction | DNA/RNA extraction |
Suggested Pairing
- Downstream Applications:
- Use with Thermo Scientific™ PCR reagents for amplification after DNA purification.
- RNA Isolation:
- Combine with TRIzol™ Reagent for efficient RNA extraction.
- Nucleic Acid Quantification:
- Validate extraction results using Thermo Scientific™ NanoDrop™ Spectrophotometer.
The Thermo Scientific™ Pierce Phenol-Chloroform + Tris Buffer offers unmatched flexibility and convenience for nucleic acid extractions, making it a reliable choice for research labs requiring precise DNA and RNA isolation.
- Pack Size: 100 mL 400 mL