Thermo Scientific™ Maxima™ Probe/ROX qPCR Master Mix (2X)

Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X) is a high-performance, ready-to-use solution optimized for probe-based real-time PCR (qPCR) applications. Formulated with Maxima Hot Start Taq DNA Polymerase, dNTPs (including dUTP), an optimized PCR buffer, and ROX passive reference dye, the mix delivers exceptional sensitivity, specificity, and reproducibility. Only template DNA and primers/probes need to be added, minimizing preparation time and reducing variability.

This 2X master mix is suitable for a wide range of applications, including gene expression analysis, siRNA validation, pathogen detection, and genotyping. The inclusion of ROX dye ensures compatibility with real-time PCR systems that require passive reference normalization.

Key Features and Benefits

• High Specificity and Sensitivity
  Maxima Hot Start Taq DNA Polymerase and an optimized buffer system minimize non-specific amplification and primer-dimer formation, allowing the detection of low-abundance targets.
• Wide Dynamic Range
  Supports accurate and linear quantification across 9 orders of magnitude.
• Reproducibility
  Pre-optimized and validated formulation ensures consistency between experiments.
• Contamination Control
  Contains dUTP for optional use with uracil-DNA glycosylase (UDG) to prevent carry-over contamination.
• Compatible with Most Instruments
  Suitable for use with real-time PCR instruments that utilize ROX normalization, including the 7000, 7300, 7700, 7900HT, StepOne™, and StepOnePlus™ systems.

Applications

• Quantitative gene expression analysis
• RNAi/siRNA validation
• Genotyping and SNP analysis
• Viral load quantification
• Pathogen detection in clinical or environmental samples

Kit Components

Specifications

Ordering Information

Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X) is a reliable and convenient solution for high-sensitivity, probe-based quantitative PCR applications. Designed to deliver precise and reproducible results, it is well-suited for research workflows that require accurate detection and quantification of nucleic acid targets across a wide dynamic range. The inclusion of ROX dye makes it compatible with a broad range of qPCR instruments, ensuring seamless integration into existing laboratory workflows.