Thermo Scientific™ Invitrogen™ Platinum™ Taq DNA Polymerase

Reliable Hot-Start Performance for Enhanced PCR Specificity

The Invitrogen™ Platinum™ Taq DNA Polymerase is a high-performance, hot-start, thermostable enzyme designed to deliver superior specificity and reproducibility in standard PCR applications. This enzyme is uniquely formulated with a thermolabile monoclonal antibody that inhibits polymerase activity until the initial denaturation step (94°C), enabling room temperature reaction setup and minimizing nonspecific amplification.

Engineered for convenience and accuracy, Platinum Taq DNA Polymerase is ideal for amplifying DNA from complex genomic, viral, and plasmid templates, offering trusted performance across various research applications.

Key Features

• Hot-start mechanism: Antibody-mediated enzyme activation at high temperatures improves specificity and reduces primer-dimer formation.
• Convenient setup: Allows assembly of PCR reactions at room temperature without risk of premature enzyme activity.
• High yield: Enhances amplification efficiency for consistent results across replicates.
• Terminal transferase activity: Adds a 3′-A overhang, compatible with TA cloning vectors.
• 5′ to 3′ exonuclease activity: Supports applications such as probe-based detection.
• No proofreading: Lacks 3′ to 5′ exonuclease activity, resulting in standard fidelity.

Applications

• Standard and hot-start PCR
• RT-PCR (with a reverse transcriptase)
• DNA amplification from:
  • Genomic DNA
  • Plasmid DNA
  • Viral DNA
• TA cloning
• Routine PCR assays in molecular biology

Note: For higher performance, faster cycling, and enhanced fidelity, consider upgrading to Platinum II Taq Hot-Start DNA Polymerase.

Specifications

Kit Contents

• Platinum Taq DNA Polymerase (120 μL)
• 10X PCR Buffer (without Mg²⁺) – 3 × 1.25 mL
• 50 mM MgCl₂ – 1 mL
• KB Extender – 1.3 mL

Ordering Information

For research use only. Not for use in diagnostic procedures.