Thermo Scientific™ Invitrogen™ Human IL-12 p70 ELISA Kit

The Thermo Scientific™ Invitrogen™ Human IL-12 p70 ELISA Kit is a robust, high-sensitivity assay designed for the quantitative detection of Interleukin-12 p70 (IL-12 p70) in human biological samples, including serum, plasma, buffered solutions, and cell culture media. This sandwich ELISA kit provides an efficient, accurate, and specific method for measuring IL-12 p70 levels, a critical cytokine in immune response regulation.

Key Features

1. High Sensitivity and Precision:
   • Analytical Sensitivity: Detects as low as 2.1 pg/mL.
   • Assay Range: 3.1–200 pg/mL.
   • Intraassay CV: 3%.
   • Interassay CV: 4.8%.
2. Versatile Sample Compatibility:
   • Suitable for human serum, plasma, and cell culture supernatants.
   • Requires only 50 µL of sample.
3. Comprehensive Kit Design:
   • Includes pre-coated 96-well plates, standards, controls, and all necessary reagents.
4. Reliable Sandwich ELISA Format:
   • Ensures specific detection of natural and recombinant human IL-12 p70 using a pre-coated capture antibody and a biotinylated detection antibody.
5. Time-Efficient Workflow:
   • Hands-on time: 1 hr 20 min.
   • Total time-to-result: 3 hr 10 min.

Applications and Use Cases

1. Immunology Research:

• Immune Response Studies:
  • Quantify IL-12 p70 to explore its role in T cell and NK cell activation.
• Cytokine Profiling:
  • Assess IL-12 p70 levels as part of broader cytokine profiling in immune disorders.

2. Clinical Research:

• Infectious Disease Monitoring:
  • Measure IL-12 p70 levels in response to bacterial, viral, or parasitic infections.
• Autoimmune Disease Studies:
  • Investigate IL-12 p70's role in modulating inflammatory responses in autoimmune conditions like rheumatoid arthritis and lupus.

3. Cancer Research:

• Therapeutic Development:
  • Evaluate IL-12 p70 as a potential therapeutic target for enhancing anti-tumor immunity.
• Tumor Microenvironment Analysis:
  • Quantify IL-12 p70 to study its role in immune modulation within the tumor microenvironment.

4. Vaccine Development:

• Adjuvant Studies:
  • Assess IL-12 p70 production as a marker for immune activation following vaccine administration.

Kit Components

Assay Principle

The Human IL-12 p70 ELISA Kit uses a sandwich ELISA method for accurate quantification:

1. Capture Phase:
   • A pre-coated antibody in the 96-well plate captures IL-12 p70 from the sample.
2. Detection Phase:
   • A biotinylated detection antibody binds to a different epitope of the IL-12 p70 molecule, forming a sandwich complex.
3. Signal Development:
   • SAV-HRP binds to the biotinylated detection antibody, and TMB substrate is added to produce a colorimetric signal.
4. Quantification:
   • Measure the optical density (OD) at 450 nm to calculate IL-12 p70 concentrations using the standard curve.

Performance Specifications

Storage and Shipping

• Storage Conditions: Store kit components at 2–8°C.
• Shipping Conditions: Shipped on wet ice to maintain reagent integrity.

Target Information: Interleukin-12 p70 (IL-12 p70)

• Function:
  • A pro-inflammatory cytokine that plays a key role in activating T cells and NK cells.
  • Stimulates the production of interferon-gamma (IFN-γ) to enhance immune responses.
• Source:
  • Produced by dendritic cells, macrophages, and B cells in response to infections or tumors.
• Clinical Significance:
  • Dysregulated IL-12 p70 levels are associated with autoimmune diseases, infectious diseases, and cancer.
• Gene Information:
  • IL12A (P35) and IL12B (P40) subunits encode the active IL-12 p70 heterodimer.

Advantages of the Kit

1. High Sensitivity and Specificity:
   • Detects low levels of IL-12 p70 with minimal cross-reactivity.
2. Validated Performance:
   • Rigorous lot-to-lot testing ensures consistent results.
3. Ease of Use:
   • Ready-to-use reagents simplify assay setup and minimize handling errors.
4. Broad Applications:
   • Suitable for immunology, cancer research, and vaccine studies.

The Thermo Scientific™ Invitrogen™ Human IL-12 p70 ELISA Kit provides a robust, sensitive, and accurate platform for quantifying IL-12 p70 in various human biological samples. Its high precision, ease of use, and broad applicability make it an essential tool for researchers investigating immune responses, disease mechanisms, and therapeutic interventions.