Thermo Scientific™ IGEPAL® CA-630

Thermo Scientific™ IGEPAL® CA-630 is a nonionic, non-denaturing detergent widely used for solubilization, isolation, and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40, a detergent that is no longer commercially available. IGEPAL CA-630 is commonly used in protein extraction, Western blotting, cell lysis, and molecular biology applications.

This detergent is effective in maintaining protein-protein and protein-lipid interactions without causing protein denaturation, making it ideal for studying protein complexes and enzyme functions.

Key Features & Benefits

1. Nonionic, Non-Denaturing Detergent

• Preserves protein activity and structure during solubilization.
• Maintains membrane protein interactions, unlike harsh denaturing detergents such as SDS.
• Chemically equivalent to Nonidet P-40, providing a suitable alternative for legacy protocols.

2. Versatile Use in Molecular & Cell Biology

• Suitable for Western blot sample preparation, immunoprecipitation, and protein purification.
• Used in cell lysis buffers to extract membrane-bound and cytoskeletal proteins.
• Compatible with enzymatic assays, RT-qPCR, and β-galactosidase (X-gal) staining experiments.

3. High-Quality & Reliable Performance

• Extensively used in research applications, ensuring consistent and reproducible results.
• May form turbidity or sediment during storage, which can be reversed by heating to 40°C.

Specifications

Applications

1. Protein Extraction & Western Blotting

• Used as a neutral detergent in homogenizing buffers for Western blotting.
• Maintains membrane protein integrity, preventing denaturation during extraction.
• Helps in cell membrane lysis without affecting antibody-antigen interactions.

2. Molecular Biology & Gene Expression Studies

• Used in X-gal staining to study lacZ gene expression in embryos.
• Compatible with RT-qPCR cell lysis buffers, preserving RNA integrity.
• Facilitates nucleic acid-protein interaction studies.

3. Cell Lysis & Biochemical Assays

• Component of cell-lysis buffers for DNA, RNA, and protein extraction.
• Used in immunoprecipitation protocols for protein complex isolation.
• Maintains enzyme activity in biochemical assays.

Storage & Handling

• Store at room temperature.
• If turbidity or sediment forms, heat to 40°C to restore clarity.
• Handle using appropriate safety precautions as per MSDS guidelines.

Thermo Scientific™ IGEPAL® CA-630 is a high-quality, nonionic detergent widely used in protein solubilization, Western blotting, and molecular biology applications. It is chemically equivalent to Nonidet P-40, making it an excellent alternative for legacy protocols. Its gentle, non-denaturing properties ensure protein integrity during cell lysis and protein purification, making it a critical reagent in biochemistry and molecular biology research.