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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Column
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Specifications:
| Application | HPLC |
| Storage Temperature | Ambient |
| Product Type | columns |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Analytical grade |
High-Efficiency, Low-Tailing Reversed-Phase Columns for LC & LC-MS
The Thermo Scientific™ Hypersil GOLD™ C18 Selectivity Columns are engineered to deliver exceptional peak shape, superior resolution, and unmatched reproducibility for modern HPLC and LC-MS applications. Manufactured using ultrapure, fully porous silica with proprietary C18 bonding and advanced multi-stage endcapping, these columns significantly reduce peak tailing—particularly for basic and polar compounds—resulting in highly reliable quantitation and consistent retention profiles.
With particle sizes available in 1.9 μm, 3 μm, 5 μm, and semi-preparative 12 μm, and a wide selection of lengths and diameters, Hypersil GOLD C18 columns offer maximum flexibility for method development, high-throughput analysis, stability studies, impurity profiling, and routine QC workflows.
Key Features
Outstanding Peak Symmetry
- Engineered to minimize silanol interactions
- Produces sharp, narrow peaks—even for difficult analytes
High Efficiency & Sensitivity
- Fully porous spherical silica for high plate counts
- Enhances LC-MS sensitivity and UV detection limits
Robust Chemical Stability
- Operational pH range: 1 to 11
- Stable performance for acidic, basic, and neutral compounds
Selectivity Versatility
- USP L1 classification (C18)
- Ideal for broad applications across pharmaceuticals, food, environment, and biochemistry
Flexible Dimensions
- Lengths: 10–250 mm
- IDs: 1.0–21.2 mm
- Particle sizes: 1.9, 3, 5, 12 μm
Applications
- Pharmaceutical API & impurity profiling
- Bioanalytical small molecule separations
- LC-MS screening workflows
- Food and environmental contaminant analysis
- High-throughput method development
- Stability-indicating assays
General Specifications
| Parameter | Value |
|---|---|
| Stationary Phase | C18 (USP L1) |
| Packing Material | Ultrapure, fully porous spherical silica |
| Endcapped | Yes |
| Pore Size | 175 Å |
| Surface Area | 220 m²/g |
| Carbon Load | ~11% |
| pH Range | 1–11 |
| Max Temperature | 60°C |
| Column Type | Reversed Phase |
| Format | Analytical & Semi-Preparative |
| Product Line | Hypersil GOLD |
| Unit Size | Each |
1. Particle Size: 5 μm
| Catalog Number | Length (mm) | ID (mm) | Particle Size |
|---|---|---|---|
| 25005-052130 | 50 | 2.1 | 5 μm |
| 25005-053030 | 50 | 3.0 | 5 μm |
| 25005-054630 | 50 | 4.6 | 5 μm |
| 25005-011001 | 10 | 1.0 | 5 μm |
| 25005-012101 | 10 | 2.1 | 5 μm |
| 25005-013001 | 10 | 3.0 | 5 μm |
| 25005-014001 | 10 | 4.0 | 5 μm |
| 25005-029223 | 20 | 21.2 | 5 μm |
| 25005-102130 | 100 | 2.1 | 5 μm |
| 25005-104630 | 100 | 4.6 | 5 μm |
| 25005-152130 | 150 | 2.1 | 5 μm |
| 25005-153030 | 150 | 3.0 | 5 μm |
| 25005-154030 | 150 | 4.0 | 5 μm |
| 25005-154630 | 150 | 4.6 | 5 μm |
| 25005-253030 | 250 | 3.0 | 5 μm |
| 25005-254630 | 250 | 4.6 | 5 μm |
2. Particle Size: 3 μm
| Catalog Number | Length (mm) | ID (mm) | Particle Size |
|---|---|---|---|
| 25003-032130 | 30 | 2.1 | 3 μm |
| 25003-034630 | 30 | 4.6 | 3 μm |
| 25003-052130 | 50 | 2.1 | 3 μm |
| 25003-053030 | 50 | 3.0 | 3 μm |
| 25003-054630 | 50 | 4.6 | 3 μm |
| 25003-101030 | 100 | 1.0 | 3 μm |
| 25003-102130 | 100 | 2.1 | 3 μm |
| 25003-103030 | 100 | 3.0 | 3 μm |
| 25003-104030 | 100 | 4.0 | 3 μm |
| 25003-104630 | 100 | 4.6 | 3 μm |
| 25003-152130 | 150 | 2.1 | 3 μm |
| 25003-153030 | 150 | 3.0 | 3 μm |
| 25003-154030 | 150 | 4.0 | 3 μm |
| 25003-154630 | 150 | 4.6 | 3 μm |
| 25003-014001 | 10 | 4.0 | 3 μm |
| 25003-011001 | 10 | 1.0 | 3 μm |
| 25003-012101 | 10 | 2.1 | 3 μm |
| 25003-013001 | 10 | 3.0 | 3 μm |
3. Particle Size: 1.9 μm (UHPLC)
| Catalog Number | Length (mm) | ID (mm) | Particle Size |
|---|---|---|---|
| 25002-012135 | 10 | 2.1 | 1.9 μm |
| 25002-022130 | 20 | 2.1 | 1.9 μm |
| 25002-031030 | 30 | 1.0 | 1.9 μm |
| 25002-032130 | 30 | 2.1 | 1.9 μm |
| 25002-051030 | 50 | 1.0 | 1.9 μm |
| 25002-052130 | 50 | 2.1 | 1.9 μm |
| 25002-053030 | 50 | 3.0 | 1.9 μm |
| 25002-054630 | 50 | 4.6 | 1.9 μm |
| 25002-101030 | 100 | 1.0 | 1.9 μm |
| 25002-102130 | 100 | 2.1 | 1.9 μm |
| 25002-103030 | 100 | 3.0 | 1.9 μm |
| 25002-152130 | 150 | 2.1 | 1.9 μm |
| 25002-202130 | 200 | 2.1 | 1.9 μm |
4. Particle Size: 12 μm (Semi-Preparative)
| Catalog Number | Length (mm) | ID (mm) | Particle Size |
|---|---|---|---|
| 25012-029223 | 20 | 21.2 | 12 μm |
| 25012-022130 | 20 | 2.1 | 12 μm |
5. Other
| Catalog Number | Length | ID | Particle Size |
|---|---|---|---|
| 25002-104630 | 100 mm | 4.6 mm | 2 μm |
Hypersil GOLD™ C18 Columns provide excellent peak symmetry, low tailing, and high efficiency for modern HPLC and LC-MS workflows. Available in 1.9, 3, 5, and 12 μm particle sizes with a broad range of lengths and diameters, they are ideal for pharmaceutical analysis, impurity profiling, environmental testing, and high-throughput research.
- Column Dimension: Particle Size: 5 μm; 50 x 2.1 mm Particle Size: 5 μm; 50 x 3 mm Particle Size: 5 μm; 50 x 4.6 mm Particle Size: 5 μm; 10 x 1.0 mm Particle Size: 5 μm; 10 x 2.1 mm Particle Size: 5 μm; 10 x 3.0 mm Particle Size: 5 μm; 10 x 4.0 mm Particle Size: 5 μm; 20 x 21.2 mm Particle Size: 5 μm; 100 x 2.1 mm Particle Size: 5 μm; 100 x 4.6 mm Particle Size: 5 μm; 150 x 2.1 mm Particle Size: 5 μm; 150 x 3.0 mm Particle Size: 5 μm; 150 x 4.0 mm Particle Size: 5 μm; 150 x 4.6 mm Particle Size: 5 μm; 250 x 3.0 mm Particle Size: 5 μm; 250 x 4.6 mm Particle Size: 3 μm; 30 x 2.1 mm Particle Size: 3 μm; 30 x 4.6 mm Particle Size: 3 μm; 50 x 2.1 mm Particle Size: 3 μm; 50 x 4.6 mm Particle Size: 3 μm; 100 x 2.1 mm Particle Size: 3 μm; 100 x 1.0 mm Particle Size: 3 μm; 100 x 3.0 mm Particle Size: 3 μm; 100 x 4.0 mm Particle Size: 3 μm; 100 x 4.6 mm Particle Size: 3 μm; 150 x 2.1 mm Particle Size: 3 μm; 150 x 3.0 mm Particle Size: 3 μm; 150 x 4.0 mm Particle Size: 3 μm; 150 x 4.6 mm Particle Size: 3 μm; 10 x 2.1 mm Particle Size: 3 μm; 10 x 1.0 mm Particle Size: 3 μm; 10 x 4.0 mm Particle Size: 3 μm; 10 x 3.0 mm Particle Size: 1.9 μm; 10 x 2.1 mm Particle Size: 1.9 μm; 20 x 2.1 mm Particle Size: 1.9 μm; 30 x 1.0 mm Particle Size: 1.9 μm; 30 x 2.0 mm Particle Size: 1.9 μm; 50 x 1.0 mm Particle Size: 1.9 μm; 50 x 2.1 mm Particle Size: 1.9 μm; 50 x 3.0 mm Particle Size: 1.9 μm; 50 x 4.6 mm Particle Size: 1.9 μm; 100 x 1.0 mm Particle Size: 1.9 μm; 100 x 2.1 mm Particle Size: 1.9 μm; 100 x 3.0 mm Particle Size: 1.9 μm; 150 x 2.1 mm Particle Size: 1.9 μm; 200 x 2.1 mm Particle Size: 12 μm; 20 x 21.2 mm Particle Size: 12 μm; 20 x 2.1 mm Particle Size: 2 μm; 100 x 4.6 mm