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Thermo Scientific™ HEPES free acid, 99+%, for molecular biology, DNase, Rnase and Protease free
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | Ambient | ||
| Product Type | Buffer | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₈H₁₈N₂O₄S |
Ultra-Pure Zwitterionic Buffer for Molecular Biology and Biochemical Applications
Thermo Scientific™ HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a high-purity, DNase-, RNase-, and Protease-free buffering agent with a minimum assay of 99.0% (titration, on dry basis). Widely used in molecular biology, biochemical research, and plant cell studies, HEPES is a zwitterionic buffer that maintains physiological pH with minimal temperature influence.
Key Features
- Molecular Biology Grade: Certified DNase-, RNase-, and protease-free
- High Purity: ≥99.0% (titration); ≤0.5% loss on drying
- Zwitterionic Buffering Agent: Ideal for use in the pH range of 6.8 to 8.2
- Stable in Solution: Excellent buffering capacity with low UV absorbance at 260/280 nm
- Reproducible Performance: Conforms to strict IR spectrum and chemical quality standards
Applications
- Cell Culture Media and Buffer Preparation
- Protein and Nucleic Acid Studies
- pH Stabilization in Enzymatic Reactions
- Plant Biology Fluorescent Labeling Probes (e.g., root cap cell studies, Tamogami et al., 2021)
- Electrophoresis and Chromatography Buffers
Specifications
| Property | Specification |
|---|---|
| CAS Number | 7365-45-9 |
| Chemical Formula | C₈H₁₈N₂O₄S |
| Molecular Weight | 238.30 g/mol |
| Appearance (Form / Color) | White crystalline powder |
| pH (0.5 M in H₂O) | 5.0 – 6.5 |
| Titration (NaOH, dry basis) | ≥99.0% |
| Loss on Drying (105°C) | ≤0.5% |
| UV Absorbance @ 260 nm | ≤0.1 |
| UV Absorbance @ 280 nm | ≤0.1 |
| Heavy Metals (as Pb) | ≤10 ppm |
| Iron (Fe) | ≤5 ppm |
| Sulfate (SO₄²⁻) | ≤500 ppm |
| Potassium (K⁺) | ≤1000 ppm |
| DNase, RNase, Protease Activity | None detected |
| Storage Temperature | Ambient |
| MDL Number | MFCD00006158 |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
Thermo Scientific™ HEPES Free Acid, 99+% provides dependable buffering performance for molecular biology workflows where enzyme and nucleic acid integrity are critical. Its chemical stability, low metal content, and lack of enzymatic contamination make it an excellent choice for pH-sensitive applications in genomics, proteomics, and cell biology.
- Pack Size: 500g 50g