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Specifications:
| Application | Microbiology, Clinical microbiology, microscopy | ||
| Storage Temperature | Ambient | ||
| Product Type | Biological Stain | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Alfa Aesar | ||
| Product Grade | Microbiology grade | Formula | C₂₀H₁₇N₃Na₂O₉S₃ |
Acid Fuchsin Sodium Salt is a synthetic triarylmethane dye used widely in histological staining, bacterial fermentation tests, and as a pH indicator. Supplied as a dark green to dark brown crystalline powder, this dye exhibits strong absorption at λmax 540–550 nm in aqueous solution and is known for its role in Masson’s Trichrome staining, where it helps differentiate muscle (red) from collagen (blue/green) in tissue sections.
This sodium salt form offers excellent solubility in water and is stable under dry, dark storage conditions. It is commonly used in cytological staining procedures, acid-base fermentation assays, and collagen detection in histology.
Key Features
- High-quality triarylmethane dye
- λmax: 540–550 nm (in water)
- Water soluble (1 mg/mL)
- Ideal for Masson’s Trichrome and Sirius Red-Acid Fuchsin protocols
- Applicable as a pH indicator in microbiological and biochemical tests
- Supplied as a crystalline powder for precise weighing and formulation
- For Research Use Only (RUO)
Chemical & Physical Properties
| Property | Value |
|---|---|
| CAS Number | 3244-88-0 |
| Molecular Formula | C₂₀H₁₇N₃Na₂O₉S₃ |
| Molecular Weight | 585.53 g/mol |
| Appearance | Dark green to dark brown powder/crystals |
| Form | Crystals or crystalline powder |
| Solubility | Soluble in water (≥1 mg/mL) |
| λmax (UV-Vis in water) | 540–550 nm |
| Storage | Cool, dry, sealed container; avoid oxidizers |
| IUPAC Name | Disodium 2-amino-5-[(4-amino-3-sulfophenyl)...] |
| Synonyms | Acid Fuchsin, Fuchsine Acid, C.I. Acid Red 1 |
| EINECS | 221-765-7 |
| Hazard Classification | Irritant (GHS07) |
Packaging Options
| Catalog No. | Quantity | Product Code |
|---|---|---|
| B22222.14 | 25 g | B22222-14 |
| B22222.22 | 100 g | B22222-22 |
| B22222.36 | 500 g | B22222-36 |
Applications
- Masson’s Trichrome staining – distinguish collagen (blue) from muscle fibers (red)
- Sirius Red–Acid Fuchsin staining for connective tissue visualization
- pH indicator in microbiological fermentation assays
- Differentiation of acid-producing bacteria
- Histochemical labeling of mucopolysaccharides and proteins
- Used in biochemical and environmental testing protocols requiring colorimetric detection
Storage & Handling
- Store in tightly sealed containers away from light and moisture
- Avoid contact with strong oxidizing agents
- Prepare aqueous solutions fresh or store at 4 °C for short periods
- For laboratory research use only; not intended for therapeutic or diagnostic applications
Supporting References
- Holman, W.L. J. Infect. Dis. 1914, 15(1), 227–233.
- Nielsen, L.F. et al. Biotechnic & Histochemistry, 1998, 73(2), 71–77.
Thermo Scientific Acid Fuchsin Sodium Salt is a reliable and high-quality dye for histological and microbiological applications. With excellent solubility and stable spectral properties, it is the reagent of choice for Masson’s Trichrome protocols, pH assays, and collagen discrimination. Suitable for use in research laboratories focusing on tissue analysis, bacteriology, and staining technique development.
- Pack Size: 100g 500g 25g