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Thermo Scientific™ Applied Biosystems™ TaqMan™ Genotyping Master Mix
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High-Performance Master Mix for SNP Genotyping and CNV Analysis
Specifications:
| Application | Gene Expression | ||
| Storage Temperature | 2-8°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Applied Biosystems™ TaqMan™ Genotyping Master Mix (2X) is a highly optimized reagent designed for real-time PCR-based SNP genotyping and copy number variation (CNV) analysis. Engineered for end-point fluorescence detection, this master mix delivers accurate allele discrimination, high call rates, and reproducible results, even with challenging genomic targets.
Validated with TaqMan™ SNP Genotyping Assays and Applied Biosystems™ real-time PCR systems, this master mix ensures reliable performance across a wide range of genotyping workflows in research and molecular biology laboratories.
Key Features
-
High Accuracy Genotyping
Produces tight, well-separated allele clusters for clear and reliable genotype calls. -
Optimized for TaqMan™ Assays
Fully validated with SNP and drug metabolism genotyping assays. -
AmpliTaq Gold™ DNA Polymerase (UP)
Ultra-pure, hot-start enzyme ensures specificity and ease of reaction setup. -
ROX™ Passive Reference Dye (Pre-mixed)
Enhances precision and signal normalization on Applied Biosystems instruments. -
Excellent Stability
Supports room temperature stability pre- and post-PCR, ideal for high-throughput workflows. -
Superior Performance with Challenging Targets
Reliable amplification of GC- and AT-rich sequences. -
High Throughput Compatibility
Suitable for large-scale genotyping studies and automated workflows.
Applications
- SNP genotyping (known and unknown variants)
- Copy number variation (CNV) analysis
- Drug metabolism studies
- Disease association studies
- Population genetics and linkage mapping
- Agricultural and breeding applications
- Candidate gene and biomarker studies
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | TaqMan™ Genotyping Master Mix |
| Brand | Applied Biosystems™ (Thermo Scientific™) |
| Catalog Number | 4371355 (example) |
| Format | 2X Master Mix |
| Polymerase | AmpliTaq Gold™ DNA Polymerase (Ultra Pure) |
| Detection Method | Primer-probe (TaqMan™) |
| Passive Reference Dye | ROX™ (pre-mixed) |
| Sample Type | Genomic DNA |
| Technique | Real-time PCR (qPCR) |
| Applications | SNP Genotyping, CNV Analysis |
| GC-Rich Performance | High |
| Reaction Speed | Standard |
| Storage Temperature | 2–8 °C |
| Shipping Condition | Wet ice |
| Quality Grade | Ultra Pure (UP) |
| Regulatory Status | For Research Use Only (RUO) |
Instrument Compatibility
Compatible with Applied Biosystems™ real-time PCR systems, including:
- StepOnePlus™ Fast Real-Time PCR System
- QuantStudio™ 3, 5, 6 Flex, 7, and 12K Flex Systems
Packaging & Ordering Information
| Catalog Number | Pack Size | Description |
|---|---|---|
| 4371353 | 1 × 1 mL | Small-scale genotyping |
| 4371355 | 1 × 10 mL | Standard pack |
| 4381656 | 2 × 10 mL | Medium throughput |
| 4371357 | 1 × 50 mL | High throughput |
| 4381657 | 2 × 50 mL | Large-scale genotyping |
Performance Highlights
- Exceptional allelic discrimination accuracy
- Consistent results across different assay types
- Supports overnight and unattended runs
- Optimized for high-throughput genotyping workflows
The Thermo Scientific™ Applied Biosystems™ TaqMan™ Genotyping Master Mix is a reliable and high-performance solution for SNP genotyping and CNV analysis. Its optimized formulation, robust enzyme system, and compatibility with TaqMan™ assays ensure accurate, reproducible results across a wide range of molecular biology applications.
- Pack Size: 1 x 1 mL, sufficient for 40 Rxns 1 x 50 mL, sufficient for 2000 Rxns 1 x 10 mL, sufficient for 400 Rxns 2 x 10 mL, sufficient for 800 Rxns 2 x 50 mL, sufficient for 4,000 Rxns