Thermo Scientific™ alamarBlue™ Dye

Thermo Scientific™ alamarBlue™ is a versatile, resazurin-based indicator dye designed to enable three key assays with a single product. It provides a reliable and sensitive means of assessing cell growth, in vitro cytotoxicity, and cytokine activity. alamarBlue™ simplifies workflows by serving multiple applications with extended stability and compatibility with various cell types and culture systems.

Key Features:

1. Versatility:
   • Perform cell viability, cytotoxicity, and cytokine assays using the same reagent.
2. Unmatched Sensitivity:
   • Accurately detects metabolic activity, even in low-cell-density samples, making it ideal for high-sensitivity assays.
3. Extended Stability:
   • Stable for up to 5 days, ensuring consistent results over prolonged assay durations.
4. Ease of Use:
   • Ready-to-use formulation allows for quick integration into laboratory protocols.
5. Non-Toxic & Non-Destructive:
   • Minimal impact on cells during analysis, enabling additional downstream applications or assays.
6. Resazurin-Based Chemistry:
   • Converts resazurin to the highly fluorescent resorufin in metabolically active cells, providing a direct measure of cellular health.

Applications:

1. Cell Viability Assays:
   • Determine cell growth and metabolic activity to assess overall cell health.
2. Cytotoxicity Assays:
   • Evaluate the toxic effects of drugs, chemicals, or other substances on cells.
3. Cytokine Assays:
   • Monitor immune response and cytokine production in vitro for immunological research.
4. Drug Screening:
   • Test the efficacy and safety of pharmaceutical compounds in preclinical studies.
5. Cancer Research:
   • Analyze tumor cell proliferation or response to therapeutic interventions.
6. Biotechnology:
   • Use in bioprocess optimization for cell-based production systems.

Specifications:

• Catalog Numbers:
  • Y00-100: 100 mL alamarBlue indicator dye.
  • Y00-025: 25 mL alamarBlue indicator dye.
  • Y00010: 10 mL alamarBlue indicator dye.
• Product Type: Indicator Dye.
• Formulation: Ready-to-use resazurin-based solution.
• Stability: Up to 5 days after application in assays.
• Unit Size: Each.

Key Benefits:

• Suitable for high-throughput screening (HTS) due to its simplicity and non-toxicity.
• Compatible with fluorescence and absorbance plate readers for flexible detection options.
• Reduces the need for multiple reagents, simplifying inventory and cost management.

How It Works:

1. Add alamarBlue™ directly to the cell culture system.
2. Incubate the cells for a specified time (as per assay protocol).
3. Measure the change in fluorescence or absorbance to assess metabolic activity and assay outcomes.

Storage and Handling:

• Store at room temperature or as per specific product guidelines.
• Avoid prolonged exposure to light to maintain reagent efficacy.

Packaging Options:

• 10 mL, 25 mL, and 100 mL volumes available to suit various laboratory scales and applications.

Research Use Only:

• alamarBlue™ is intended for research use and is not for diagnostic or therapeutic procedures.