Thermo Scientific™ 5-Methylphenazinium methyl sulphate ≥98%

5-Methylphenazinium methyl sulphate (PMS) is a water-soluble redox dye frequently used as an electron carrier in various biochemical assays, especially in conjunction with tetrazolium salts like MTT, INT, or XTT. It is commonly employed in NADH/NADPH-coupled dehydrogenase activity assays, where PMS transfers electrons from the reduced coenzyme to the colorimetric reagent, producing a measurable colored formazan product.

Thermo Scientific supplies PMS at ≥98% purity, with stringent specifications including UV spectrophotometry (≥97.5%), GC purity (≥96%), and a titration assay of ≥99% (on dry basis). It is delivered as a dark yellow to brown crystalline powder and requires freezer storage for optimal stability.

Key Features

• ≥98% purity (Titration ≥99%)
• Strong electron carrier in redox reactions
• Used in dehydrogenase assays and cell viability tests
• Soluble in water; lambda max at 387.5 nm
• Passes HPLC suitability and membrane filtration tests
• Stable, crystalline solid with excellent shelf life
• GHS07 – Irritant; use appropriate PPE
• Requires cold storage (freezer)

Applications

• Electron transport mediator in biochemical assays
• NADH/NADPH-dependent dehydrogenase activity detection
• Commonly used with MTT, XTT, INT and similar dyes
• Colorimetric assays for enzyme kinetics and metabolic activity
• High-sensitivity tests in microbiology, cell biology, and diagnostics
• Used in membrane filtration quality control
• HPLC suitability testing in analytical chemistry

Chemical & Physical Properties

Available Packaging

Thermo Scientific™ 5-Methylphenazinium Methyl Sulphate (PMS), 98% is a reliable, high-purity redox mediator essential for colorimetric detection of enzymatic activity. Its role as an electron shuttle in metabolic assays makes it ideal for use in biochemical research, diagnostics, and high-throughput screening, offering precise, reproducible results in enzyme activity and cell viability studies.